A novel oxazole-containing tetraene compound, JBIR-159, produced by heterologous expression of the cryptic trans-AT type polyketide synthase biosynthetic gene cluster
A novel oxazole-containing tetraene compound, JBIR-159, produced by heterologous expression of the cryptic trans-AT type polyketide synthase biosynthetic gene cluster
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DOI：
10.1038/s41429-021-00410-9
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发表时间：
2021-02-08
影响因子：
3.3
通讯作者：
Shin-ya, Kazuo
中科院分区：
文献类型：
--
作者：
Hashimoto, Takuya;Hashimoto, Junko;Shin-ya, Kazuo
利用基因组挖掘技术，我们从Streptomyces versipellis 4083-SVS 6中发现了一个新的含有反式AT型PKS基因的生物合成基因簇。从细菌人工染色体（BAC）文库中获得了包含整个生物合成基因簇的细菌人工染色体（BAC）克隆pKU503JL68_PN1_P10-C12。变铅青链霉菌TK 23中生物合成基因簇的异源表达导致了一种新的多烯化合物JBIR-159的产生。本文报道了JBIR-159的生物合成基因簇、异源表达、分离、结构测定和简要的生物活性。
Using genome mining approach, we identified a novel biosynthetic gene cluster containing trans-AT type PKS genes from Streptomyces versipellis 4083-SVS6. A bacterial artificial chromosome (BAC) clone, pKU503JL68_PN1_P10-C12, accommodating the entire biosynthetic gene cluster was obtained from a BAC library. Heterologous expression of the biosynthetic gene cluster in Streptomyces lividans TK23 led to the production of a novel polyene compound, JBIR-159. We report herein the biosynthetic gene cluster for JBIR-159, and the heterologous expression, isolation, structure determination and a brief biological activity.