Isolation and structural characterization of epilancin 15X, a novel lantibiotic from a clinical strain of Staphylococcus epidermidis
Isolation and structural characterization of epilancin 15X, a novel lantibiotic from a clinical strain of Staphylococcus epidermidis
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DOI：
10.1016/j.febslet.2005.01.083
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发表时间：
2005-03-28
期刊：
影响因子：
3.5
通讯作者：
van Nuland, NAJ
中科院分区：
文献类型：
--
作者：
Ekkelenkamp, MB;Hanssen, M;van Nuland, NAJ
羊毛硫抗生素作为食品防腐剂和最近作为抗生素的潜在应用极大地增加了对这些抗菌肽的兴趣。在这里，我们报告的主要和三维结构的新lantibiotic epilancin 15 X从表皮葡萄球菌使用高分辨率核磁共振光谱和串联质谱的阐明。该分子含有10个后修饰的氨基酸，三个lanthirons环结构和一个羟基-丙酰基N-末端部分。一级和三级结构以及正电荷的分布与先前鉴定的羊毛硫抗生素epilancin K7非常相似，最有可能表明共同的作用模式。(c)2005年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
The potential application of lantibiotics as food-preserving agents and more recently as antibiotics has strongly increased the interest in these antibacterial peptides. Here, we report the elucidation of the primary and three-dimensional structures of the novel lantibiotic epilancin 15X from Staphylococcus epidermidis using high-resolution nuclear magnetic resonance spectroscopy and tandem mass spectrometry. The molecule contains ten post-translationally modified amino acids, three lanthionine ring structures and a hydroxy-propionyl N-terminal moiety. The primary and tertiary structure and the distribution of positive charges are closely similar to the previously identified lantibiotic epilancin K7, most likely indicative of a common mode of action. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.