Rapid preparation of a panel of polyclonal antibodies to Drosophila segmentation proteins
Rapid preparation of a panel of polyclonal antibodies to Drosophila segmentation proteins
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DOI：
10.1007/s004270050184
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发表时间：
1998-07-01
影响因子：
2.4
通讯作者：
Reinitz, J
中科院分区：
文献类型：
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作者：
Kosman, D;Small, S;Reinitz, J
我们描述了一种从细菌表达的蛋白质中快速产生一组高质量多克隆抗体的方法。每种蛋白质需要大约1 2/3天的制备时间。加速该过程的一个步骤是通过沉淀的十二烷基硫酸钠（SDS）使纯化的条带可视化。纯化的重组蛋白的抗原性可以通过在双蒸水中沉淀来增加。显示了使用获得的血清对果蝇胚胎进行荧光染色的结果。
We describe a method for rapidly raising a panel of high quality polyclonal antibodies from bacterially expressed proteins. Approximately 1 2/3 days of preparation is required per protein. One step that speeds up the procedure is the visualization of purified bands by precipitated sodium dodecyl sulfate (SDS). Antigenicity of the purified recombinant proteins may be increased by precipitation in double-distilled water. The results of using the serums obtained for fluorescent staining of Drosophila embryos are shown.