Kaempferol Glycosides with Antioxidant Activity from Brassica juncea
Kaempferol Glycosides with Antioxidant Activity from Brassica juncea
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DOI：
10.1007/s12272-009-2006-3
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发表时间：
2009-10-01
影响因子：
6.7
通讯作者：
Choi, Jae Sue
中科院分区：
文献类型：
--
作者：
Jung, Hyun Ah;Woo, Ju Jung;Choi, Jae Sue
从芥菜型油菜（Brassica juncea）叶中分离得到三种山奈酚苷，山奈酚-3-O-（2-O-芥子酰基）-β-D-吡喃葡萄糖基-（1 -> 2）-β-D-吡喃葡萄糖苷-7-O-β-D-吡喃葡萄糖苷（1），山奈酚-3-O-β-D-吡喃葡萄糖基-（1 -> 2）-β-D-吡喃葡萄糖苷-7-O-β-D-吡喃葡萄糖基-（1 -> 6）-β-D-吡喃葡萄糖苷（2），分离得到3-O-（2-O-芥子酰基）-β-D-吡喃葡萄糖基-（1 -> 2）-β-D-吡喃葡萄糖苷-7-O-β-D-吡喃葡萄糖基-（1 -> 6）-β-D-吡喃葡萄糖苷（3）。抗氧化活性通过测定对1，1-二苯基-2-苦肼基（DPPH）自由基和过氧亚硝酸根（ONOO-）的清除能力来测定。化合物1和3显示出良好的抗氧化活性，对DPPH的IC 50值分别为28.61和36.93 μ M;对ONOO-的IC 50值分别为9.79和11.40 μ M。然而，化合物2显示没有DPPH清除活性和弱的ONOO-清除活性，IC 50值为32.00 μ M。
From the leaves of Brassica juncea, three kaempferol glycosides, kaempferol-3-O-(2-O-sinapoyl)-beta-D-glucopyranosyl-(1 -> 2)-beta-D-glucopyranoside-7-O-beta-D-glucopyranoside (1), kaempferol-3-O-beta-D-glucopyranosyl-(1 -> 2)-beta-D-glucopyranoside-7-O-beta-D-glucopyranosyl-(1 -> 6)-beta-D-glucopyranoside (2), and kaempferol-3-O-(2-O-sinapoyl)-beta-D-glucopyranosyl-(1 -> 2)-beta-D-glucopyranoside-7-O-beta-D-glucopyranosyl-(1 -> 6)-beta-D-glucopyranoside (3) were isolated and the structures elucidated on the basis of spectral and chemical evidences. Antioxidant activities were determined by measuring the scavenging activities on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and peroxynitrite (ONOO-). Compounds 1 and 3 showed good antioxidant activities with respective IC50 values of 28.61 and 36.93 mu M toward DPPH; respective IC50 values of 9.79 and 11.40 mu M toward ONOO-. However, compound 2 showed no DPPH scavenging activity and weak ONOO- scavenging activity with an IC50 value of 32.00 mu M.