Gene amplification of Myc and its coamplification with ERBB2 and EGFR in gallbladder adenocarcinoma.
Gene amplification of Myc and its coamplification with ERBB2 and EGFR in gallbladder adenocarcinoma.
复制标题
DOI：
--
复制
发表时间：
2009
影响因子：
2
通讯作者：
A. Ooi;Shioto Suzuki;K. Nakazawa;J. Itakura;I. Imoto;Hiroyuki Nakamura;Y. Dobashi
中科院分区：
文献类型：
--
作者：
A. Ooi;Shioto Suzuki;K. Nakazawa;J. Itakura;I. Imoto;Hiroyuki Nakamura;Y. Dobashi
背景在共扩增基因的组合中，Myc和ERBB 2或EGFR因其与细胞遗传学、肿瘤发生和肿瘤治疗的相关性而引起了广泛的关注。材料与方法应用荧光原位杂交技术（FISH）检测97例胆囊癌组织中Myc、ERBB 2和EGFR基因的扩增情况。结果FISH显示3例肿瘤有Myc扩增。双色荧光原位杂交结果显示，其中1例有两个肿瘤细胞群，扩增的Myc与ERBB 2、Myc与EGFR共存。第二个肿瘤具有高水平的Myc扩增，而没有ERBB 2或EGFR扩增。第三个肿瘤具有低水平的Myc和ERBB 2共扩增。1例8型多体性也显示ERBB 2和EGFR扩增。结论Myc基因扩增引起的基因组不稳定性可能导致EGFR和/或ERBB 2的特异性扩增。
BACKGROUND Among the combinations of coamplified genes, Myc and ERBB2 or EGFR have attracted much attention for their relevance to cytogenetics, carcinogenesis and cancer therapy. MATERIALS AND METHODS Gene amplification of Myc, ERBB2 and EGFR were examined on 97 formalin-fixed and paraffin-embedded gallbladder carcinomas, by fluorescence in situ hybridization (FISH). RESULTS FISH revealed three tumors had Myc amplification. By dual color FISH, one of these three tumors had two populations of tumor cells with the coexistence of amplified Myc and ERBB2, and Myc and EGFR. The second tumor had a high level of amplification of Myc without amplification of ERBB2 or EGFR. The third tumor had a low level of coamplification of Myc and ERBB2. A case of polysomy 8 also demonstrated amplification of ERBB2 and EGFR. CONCLUSION The present study shows that genomic instability due to Myc amplification may cause specific amplification of EGFR and/or ERBB2.