CONCOMITANT EXPRESSION OF HEPATOCYTE GROWTH-FACTOR (HGF), HGF ACTIVATOR AND C-MET GENES IN HUMAN GLIOMA-CELLS IN-VITRO
CONCOMITANT EXPRESSION OF HEPATOCYTE GROWTH-FACTOR (HGF), HGF ACTIVATOR AND C-MET GENES IN HUMAN GLIOMA-CELLS IN-VITRO
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DOI：
10.1016/0014-5793(95)00949-a
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发表时间：
1995-09-18
期刊：
影响因子：
3.5
通讯作者：
KOONO, M
中科院分区：
文献类型：
--
作者：
MORIYAMA, T;KATAOKA, H;KOONO, M
建立了3个体外共表达肝细胞生长因子(HGF)及其受体c-Met基因的人脑胶质母细胞瘤NEM细胞系，逆转录/聚合酶链式反应(RT-PCR)研究表明，该细胞还表达了HGF激活物(HGF激活物)基因，提示HGF可能作为自分泌因子在体外培养的胶质瘤细胞中发挥作用，激活物基因在其他脑胶质瘤细胞系、胶质瘤组织和正常脑组织中也可检测到，HGF、HGF激活物和c-Met的同时表达，在5例胶质母细胞瘤中也有1例体内检测到。
Three nem cell lines of human glioblastoma have been established, These cells co-expressed hepatocyte growth factor (HGF) and its receptor, c-Met, genes in vitro, Reverse-transcriptase/polymerase-chain reaction study revealed that the cells also expressed gene for HGF activator, a recently cloned serine proteinase, suggesting that HGF might have a role in glioma cells in vitro as an autocrine factor, The activator mRNA was also detected in other well-established glioma cell lines, glioma tissues and normal brain, The concomitant expression of HGF, HGF activator and c-met,vas also detected in one glioblastoma case in vivo out of five tested.