Differentially expressed circulating microRNAs associated with idiopathic recurrent pregnancy loss
Differentially expressed circulating microRNAs associated with idiopathic recurrent pregnancy loss
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DOI：
10.1016/j.gene.2020.145334
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发表时间：
2021-02-05
期刊：
影响因子：
3.5
通讯作者：
Almawi, Wassim Y.
中科院分区：
文献类型：
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作者：
Jairajpuri, Deeba S.;Malalla, Zainab H.;Almawi, Wassim Y.
复发性妊娠丢失（RPL）是一种主要的妊娠并发症，据报道影响所有妊娠的2-3%。目前，RPL缺乏有效的治疗方法和可靠的诊断和预后生物标志物。循环microRNA最近被描述为妊娠相关并发症的潜在生物标志物。本研究的目的是确定RPL患者血浆中microRNA的表达模式，作为RPL的潜在早期生物标志物。研究对象包括20名早期RPL妇女（妊娠8-12周流产）和20名年龄和妊娠匹配的经产对照妇女。从母亲血浆中提取循环microRNA，并使用定制的路径聚焦miRNA分析试剂盒测定差异microRNA表达。与对照组相比，在10种差异表达的microRNA中，RPL患者中Hsa-let-7 e、Hsa-miR-221- 3 p、Hsa-miR-16、Hsa-miR-519 d、Hsa-miR-184、Hsa-miR-410上调，而Hsa-miR-21、Hsa-miR-125、Hsa-let-7a、Hsa-let-7 d下调。其中，5种新的microRNA首次被报道与RPL相关。其中Hsa-let-7 e、Hsa-miR-519 d、Hsa-miR-410在RPL中表达上调，Hsa-let-7a、Hsa-let-7 d在RPL中表达下调。虽然其与RPL的相关性在早期就有报道，但这项研究也是首次报道循环液中Hsa-miR-184的上调与RPL相关。该研究有助于了解RPL中循环microRNA的表达模式，其可能参与其发病机制，并证明其作为RPL的非侵入性诊断和预后生物标志物的潜在作用。
Recurrent pregnancy loss (RPL) is a major pregnancy complication which reportedly affects 2-3% of all pregnancies. Currently, RPL lacks an effective therapy and a reliable diagnostic and prognostic biomarker. Circulating microRNAs were recently described as potential biomarkers of pregnancy-associated complications. The aim of this study was to determine microRNA expression patterns in the plasma of RPL patients as potential early biomarker of RPL. Study subjects comprised 20 women with early RPL (miscarriage at 8-12 weeks of gestation), and 20 age- and gestation-matched multiparous control women. Circulating microRNAs were extracted from maternal plasma, and the differential microRNA expression were determined using customized pathway-focused miRNA profiler kit. Of the 10 differentially-expressed microRNAs identified, Hsa-let-7e, Hsa-miR-221-3p, Hsa-miR-16, Hsa-miR-519d, Hsa-miR-184, Hsa-miR-410 were upregulated, while Hsa-miR-21, Hsa-miR-125, Hsa-let-7a, Hsa-let-7d were downregulated in RPL cases as compared to control women. Of these, 5 novel microRNAs were reported for the first time to be associated with RPL. These comprised Hsa-let-7e, Hsa-miR-519d, Hsa-miR-410 which were upregulated, and Hsa-let-7a, Hsa-let-7d which were downregulated in RPL. While its association with RPL was reported earlier, this study is also the first to report on the upregulation of Hsa-miR-184 in circulating fluids in association with RPL. The study provides for understanding circulating microRNAs expression pattern in RPL which may be involved in its pathogenesis and demonstrates their potential role as noninvasive diagnostic and prognostic biomarkers for RPL.