Normolipidemic subjects with low HDL cholesterol levels have altered HDL subpopulations.
Normolipidemic subjects with low HDL cholesterol levels have altered HDL subpopulations.
复制标题
高密度脂蛋白胆固醇水平低的血脂正常受试者的高密度脂蛋白亚群发生了改变。
DOI：
10.1161/01.atv.17.10.1885
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Roheim,PS
中科院分区：
文献类型：
--
作者：
Asztalos,BF;Lefevre,M;Foster,TA;Tulley,R;Windhauser,M;Wong,L;Roheim,PS
流行病学研究已经证实，即使在没有增加低密度脂蛋白胆固醇水平的情况下，血浆高密度脂蛋白浓度也与患冠心病的风险呈负相关。我们推测，特定的高密度脂蛋白亚群可能与高密度脂蛋白的抗动脉粥样硬化特性有关。采用双向凝胶电泳法对79例血脂正常的健康男性受试者的高密度脂蛋白亚群进行定量。为了消除饮食的影响，志愿者们在6周的时间里平均食用美国人的饮食。饮食结束后，根据受试者的高密度脂蛋白胆固醇水平将受试者分为低高密度脂蛋白胆固醇/L组(35 mg/dL)、中高密度脂蛋白胆固醇/L组(35<；50 mg/dL)和高≥组(≥50 mg/dL)。血浆甘油三酯和胰岛素水平在正常范围内，但低密度脂蛋白胆固醇水平的受试者血浆甘油三酯和胰岛素浓度高于中等或高密度脂蛋白胆固醇水平的受试者。低α-C组最大迁移高密度脂蛋白亚群(α-1)载脂蛋白A-I的绝对浓度(mg/dL)低于中、高密度脂蛋白胆固醇组(P<0.01)。α1的载脂蛋白A-I的相对浓度(百分比分布)降低(P<0.01)，α3亚群的相对浓度(百分比分布)增加(P<0.01)。高密度脂蛋白胆固醇与α-1呈正相关，与α-3呈负相关。α1和α3之间的apoA-I分布(相对浓度)呈负相关，表明α1和α3亚群之间存在相互转化，可能是通过胆固醇酯转移蛋白。β前亚群表现出与高密度脂蛋白胆固醇相反的趋势，而α前亚群的行为与α迁移亚群相似。载脂蛋白A-I和载脂蛋白A-II颗粒在不同的高密度脂蛋白亚群中的共存表明，α1、前β1和前β2亚群是只有apoA-I的颗粒，而不是apoA-I：A-II颗粒。
Epidemiological studies have established that plasma concentration of HDL is inversely correlated with the risk of coronary heart disease, even in the absence of increased LDL cholesterol levels. We postulate that specific HDL subpopulations may be responsible for antiatherogenic properties of HDL. HDL subpopulations were quantitated by two-dimensional gel electrophoresis in 79 normolipidemic healthy male subjects. To eliminate the influence of diet, volunteers consumed an average American diet for 6 weeks. After the diet period, subjects were stratified according to their HDL cholesterol (HDL-C) levels to low HDL-C <0.91 mmol/L (<35 mg/dL), medium >0.91<1.30 mmol/L (>35 <50 mg/dL), and high ≥1.30 mmol/L (≥50 mg/dL) groups. Plasma triglycerides and insulin levels were in the normal range, but subjects with low HDL-C levels had higher concentrations of plasma triglycerides and insulin than subjects with medium or high HDL-C concentrations. The absolute concentration (mg/dL) of apoA-I in the largest α-migrating HDL subpopulation (α1) was (P<.01) lower in the low HDL-C subjects compared with the medium and high HDL-C groups. The relative concentration (percent distribution) of apoA-I was decreased (P<.01) in α1and increased (P<.01) in α3subpopulations. A positive correlation between HDL-C and α1(P<.001) and a negative correlation between HDL-C and α3were observed. The inverse correlation of apoA-I distribution (relative concentration) between α1and α3suggests an interconversion of α1and α3subpopulations, possibly by cholesteryl ester transfer protein. Pre-β subpopulations showed an inverse trend with HDL-C, while the pre-α subpopulation behaved similarly to the α-migrating subpopulation. Colocalization of apoA-I and apoA-II particles in the different HDL subpopulations demonstrated that α1, pre-β1, and pre-β2subpopulations are apoA-I–only particles rather than apoA-I:A-II particles.