Antigen processing and presentation by glomerular visceral epithelium in vitro.
Antigen processing and presentation by glomerular visceral epithelium in vitro.
复制标题
体外肾小球内脏上皮的抗原加工和呈递。
DOI：
10.1038/ki.1991.9
复制
发表时间：
1991
影响因子：
19.6
通讯作者：
Rennke,HG
中科院分区：
文献类型：
--
作者：
Mendrick,DL;Kelly,DM;Rennke,HG
肾小球内脏上皮抗原的体外加工和提呈。虽然巨噬细胞被认为是抗原呈递细胞（APC）的原型，但最近的研究强调了几种实质细胞和间充质细胞在这一过程中的潜在作用。我们研究了培养的肾小球内脏上皮细胞（GEC）作为有效APC的能力，并将这种能力与腹膜巨噬细胞所证明的能力进行了比较。亲和纯化和体外繁殖的大鼠GEC暴露于鸡蛋溶菌酶、锁眼帽贝血青素和阳离子铁蛋白中。作为效应细胞，我们使用抗原特异性T细胞杂交瘤；通过测定组织培养上清液中白细胞介素2 （IL-2）的水平来评估抗原呈递水平。细胞因子处理的GEC能够以剂量依赖的方式处理和呈递所有抗原。抗原呈递的关键是细胞内的抗原加工和la在细胞表面的存在。我们的研究结果表明，GEC可以作为有效的APC，并进一步表明这种能力可能与体内肾小球毛细血管水平的细胞介导的免疫损伤有关。
Antigen processing and presentation by glomerular visceral epithelium in vitro. Although macrophages are considered the prototype of antigen presenting cells (APC), recent studies have emphasized the potential role of several parenchymal and mesenchymal cells in this process. We have studied the capacity of cultured glomerular visceral epithelial cells (GEC) to act as effective APC and compared this capacity with that demonstrated by peritoneal macrophages Affinity-purified and in vitro propagated rat GEC were exposed to hen egg lysozyme, keyhole limpet hemocyanin, and cationic ferritin. As effector cells, we used antigen-specific T cell hybridomas; the level of antigen presentation was assessed by determining the level of interleukin 2 (IL-2) present in tissue culture supernatants Cytokine-treated GEC were capable of processing and presenting all antigens in a dose-dependent manner. Crucial for antigen presentation were intracellular processing of antigen and the presence of la on the cell surface. Our findings indicate that GEC can act as effective APC, and further suggest that this capacity may be relevant to cell-mediated immune injury at the level of the glomerular capillaries in vivo.