Transgenic engineering of male-specific muscular hypertrophy
Transgenic engineering of male-specific muscular hypertrophy
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DOI：
10.1073/pnas.0502426102
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发表时间：
2005-05-03
影响因子：
11.1
通讯作者：
Georges, M
中科院分区：
文献类型：
--
作者：
Pirottin, D;Grobet, L;Georges, M
使用一个两步的程序，包括插入基因打靶和重组酶介导的盒交换ES细胞，我们已经产生了两个行的转基因小鼠表达显性负潜伏期相关的肌肉生长抑制素前肽的肌球蛋白轻链1F启动子和1/3增强子的控制下，从Y染色体上的TSPY基因座。相应品系的雄性的特征在于骨骼肌质量增加5-20%。本实验证明了结合公牛的上级产肉能力和奶牛的产奶能力的更有效的牛生产系统的可行性。
Using a two-step procedure involving insertional gene targeting and recombinase-mediated cassette exchange in ES cells, we have produced two lines of transgenic mice expressing a dominant-negative latency-associated myostatin propeptide under control of the myosin light chain 1F promoter and 1/3 enhancer from the TSPY locus on the Y chromosome. Males of the corresponding lines are characterized by a 5-20% increase in skeletal muscle mass. This experiment demonstrates the feasibility of a more efficient cattle production system combining superior beef production abilities for bulls and dairy abilities for cows.