DMSO enhances electrospray response, boosting sensitivity of proteomic experiments
DMSO enhances electrospray response, boosting sensitivity of proteomic experiments
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DOI：
10.1038/nmeth.2610
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发表时间：
2013-10-01
期刊：
影响因子：
48
通讯作者：
Kuster, Bernhard
中科院分区：
文献类型：
--
作者：
Hahne, Hannes;Pachl, Fiona;Kuster, Bernhard
我们报告称，液相色谱溶剂中低含量的二甲基亚砜 (DMSO) 会显着增强肽的电喷雾电离，从而将自下而上蛋白质组学中蛋白质鉴定的灵敏度提高十倍。该方法可以在任何 LC-MS/MS 系统上轻松实施，无需修改硬件或软件，也无需额外成本。
We report that low percentages of dimethylsulfoxide (DMSO) in liquid chromatography solvents lead to a strong enhancement of electrospray ionization of peptides, improving the sensitivity of protein identification in bottom-up proteomics by up to tenfold. The method can be easily implemented on any LC-MS/MS system without modification to hardware or software and at no additional cost.