Self-assembled Mn-doped ZnSe quantum dot–methyl viologen nanohybrids as an OFF–ON fluorescent probe for time-resolved fluorescence detection of tiopronin
Self-assembled Mn-doped ZnSe quantum dot–methyl viologen nanohybrids as an OFF–ON fluorescent probe for time-resolved fluorescence detection of tiopronin
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DOI：
10.1039/c3ay40907d
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发表时间：
2013-08
影响因子：
3.1
通讯作者：
Dong Zhu;Wei Li;H. Wen;Jianrong Zhang;Jun‐Jie Zhu
中科院分区：
文献类型：
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作者：
Dong Zhu;Wei Li;H. Wen;Jianrong Zhang;Jun‐Jie Zhu
由于复杂的样品成分、缓冲液和裂解液的高背景噪声，常规均相荧光法分析准确度和S(信噪比)的提高一直受到限制。本文提出了一种自组装的锌硒量子点-甲基紫精纳米杂化荧光探针，用于硫普罗宁的时间分辨荧光检测，具有较高的S/氮比和选择性，检测下限为0.1molM L−1，线性响应范围为4~160μM，有望在灵敏生物传感和药物分析中得到应用。
The improvement of analytical accuracy and S/N (signal to noise ratio) based on conventional homogeneous fluorometry was always limited because of high background noise from complicated sample ingredients, buffer solution and lysates. Herein, a self-assembled Mn-doped ZnSe quantum dot–methyl viologen nanohybrid as an OFF–ON fluorescent probe is presented for the time-resolved fluorescence detection of tiopronin, showing high S/N and selectivity with a low detection limit of 0.1 μmol L−1 and a linear response ranging from 4 to 160 μM. We anticipate that this probe will have promising applications for sensitive biosensing and drug analysis.