Rising maternal circulating GH during murine pregnancy suggests placental regulation
Rising maternal circulating GH during murine pregnancy suggests placental regulation
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DOI：
10.1530/ec-17-0032
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发表时间：
2017-05-01
影响因子：
2.9
通讯作者：
Chen, Chen
中科院分区：
文献类型：
--
作者：
Gatford, Kathryn L.;Muhlhausler, Beverly S.;Chen, Chen
在人类中，胎盘衍生的激素包括生长激素(GH)有助于母体对怀孕的适应，在几个物种中，间歇性的母体GH给药增加了胎儿的生长。循环生长激素的类型和丰富程度对其活性都很重要，但它们在怀孕期间的变化仅在人类和大鼠中有报道。本研究的目的是描述未怀孕雌性小鼠和整个小鼠妊娠期间生长激素的循环和分泌特征。测定了非妊娠间情期雌性C57BL/6J小鼠(n=9)、妊娠雌性C57BL/6J小鼠(n=9)、妊娠雌性C57BL/6J小鼠(n=7)交配后第8.5~9.5天(n=8)、妊娠中期(n=7)和交配后17.5天(n=7)外周血中生长激素的浓度。用去卷积分析确定GH分泌的动力学和分泌模式，用近似熵分析计算GH系列浓度的有序性和规律性。各组循环生长激素呈搏动性变化。平均循环GH、总GH分泌率和基础GH分泌率从妊娠早期到中期显著升高，然后持续升高。两组间的脉搏频率和脉动生长激素分泌速率相似。各孕鼠GH脉冲的不规则性均高于未孕小鼠。小鼠妊娠中循环GH的增加与这种激素在母体适应妊娠和胎盘发育中的重要作用是一致的。妊娠中期基础分泌量增加的时间，与绒毛尿囊膜胎盘的形成和母体血液流经胎盘的开始同步，表明胎盘衍生因子对脑垂体分泌的调节。
Placenta-derived hormones including growth hormone (GH) in humans contribute to maternal adaptation to pregnancy, and intermittent maternal GH administration increases foetal growth in several species. Both patterns and abundance of circulating GH are important for its activity, but their changes during pregnancy have only been reported in humans and rats. The aim of the present study was to characterise circulating profiles and secretory characteristics of GH in non-pregnant female mice and throughout murine pregnancy. Circulating GH concentrations were measured in whole blood (2 mu L) collected every 10 min for 6 h in non-pregnant diestrus female C57Bl/6J mice (n = 9), and pregnant females at day 8.5-9.5 (early pregnancy, n = 8), day 12.5-13.5 (mid-pregnancy, n = 7) and day 17.5 after mating (late pregnancy, n = 7). Kinetics and secretory patterns of GH secretion were determined by deconvolution analysis, while orderliness and regularity of serial GH concentrations were calculated by approximate entropy analysis. Circulating GH was pulsatile in all groups. Mean circulating GH and total and basal GH secretion rates increased markedly from early to mid-pregnancy, and then remained elevated. Pulse frequency and pulsatile GH secretion rate were similar between groups. The irregularity of GH pulses was higher in all pregnant groups than that in non-pregnant mice. Increased circulating GH in murine pregnancy is consistent with an important role for this hormone in maternal adaptation to pregnancy and placental development. The timing of increased basal secretion from mid-pregnancy, concurrent with the formation of the chorioallantoic placenta and initiation of maternal blood flow through it, suggests regulation of pituitary secretion by placenta-derived factors.