Levels of transient gap junctions between the retinal pigment epithelium and the neuroblastic retina are influenced by catecholamines and correlate with patterns of cell production.
Levels of transient gap junctions between the retinal pigment epithelium and the neuroblastic retina are influenced by catecholamines and correlate with patterns of cell production.
复制标题
视网膜色素上皮和神经母细胞视网膜之间的瞬时间隙连接水平受儿茶酚胺的影响，并与细胞生成模式相关。
DOI：
10.1002/cne.21388
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Tibber MS
中科院分区：
文献类型：
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作者：
Tibber MS
视网膜有丝分裂发生在视网膜色素上皮（RPE）和神经视网膜之间的界面处。多项研究强调了间隙连接介导的通讯在调节视网膜器官发生中发挥的重要作用。在此，在视网膜细胞产生的主要阶段期间，在大鼠体内在视网膜和RPE之间的界面处检查差距连接蛋白连接蛋白43的定位模式和功能。连接蛋白43表达在这个网站从E15起，水平随后进行时间调节。当Cx43蛋白水平实验性降低时，通过使用反义寡核苷酸，视网膜中的有丝分裂活性显著降低。相反，在色素减退的眼中，有丝分裂水平升高与连接蛋白43的显著增加相关。过量的蛋白质水平和升高的有丝分裂都通过体内施用L-DOPA（黑色素合成途径中的多巴胺前体和中间化合物）来校正。这些发现表明，连接蛋白43介导的视网膜和RPE之间的通信对于视网膜器官发生的正确起搏至关重要。此外，这一途径可能是门控水平的眼儿茶酚胺。J. Comp.神经元503：128-134，2007.© 2007 Wiley利斯公司
Retinal mitosis takes place at the interface between the retinal pigment epithelium (RPE) and the neural retina. Multiple studies have highlighted the essential role that gap junction‐mediated communication plays in the regulation of retinal organogenesis. Here, the localization pattern and function of the gap junction protein connexin 43 were examined in vivo in the rat at the interface between the retina and RPE during the main phases of retinal cell production. Connexin 43 was expressed at this site from E15 onward, and levels were subsequently temporally regulated. When Cx43 protein levels were reduced experimentally, by using antisense oligodeoxynucleotides, mitotic activity in the retina decreased significantly. Conversely, in the hypopigmented eye elevated mitotic levels were associated with a significant increase of connexin 43. Both excess protein levels and elevated mitosis were corrected by the in vivo administration of L‐DOPA (a dopamine precursor and intermediary compound in the melanin synthesis pathway). These findings suggest that connexin 43‐mediated communication between the retina and RPE is essential for the correct pacing of retinal organogenesis. Furthermore, this pathway may be gated by levels of ocular catecholamines. J. Comp. Neurol. 503:128–134, 2007. © 2007 Wiley‐Liss, Inc.