Progress in the direct structural characterization of fibrous amphiphilic supramolecular assemblies in solution by transmission electron microscopic techniques
Progress in the direct structural characterization of fibrous amphiphilic supramolecular assemblies in solution by transmission electron microscopic techniques
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DOI：
10.1016/j.cis.2014.01.007
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发表时间：
2014-06-01
影响因子：
15.6
通讯作者：
Boettcher, Christoph
中科院分区：
文献类型：
--
作者：
Berlepsch, Hans V.;Ludwig, Kai;Boettcher, Christoph
由于各种当前和有前景的技术应用，两亲性分子自组装成纤维结构在过去几十年中一直是众多研究的主题。尽管它们的头基化学性质非常不同，但许多源自碳水化合物、羰花青染料或氨基酸的天然和合成两亲性化合物倾向于通过在水中的分子自组装形成纤维结构，主要是扭曲的带或管。通常会观察到这些组装结构之间的转变，这是 Wolfgang Helfrich 已经在理论上解决的现象，并且仍然是当前研究的焦点。随着合适的样品制备和电子光学成像技术的发展，低温透射电子显微镜（cryo-TEM）与三维（3D）重建技术相结合已成为超分子组装体特别流行的直接表征技术。在这里，我们回顾了从特别是纤维结构（优选在三个维度）的冷冻 TEM 数据中获取精确结构信息的最新进展。 (c) 2014 Elsevier B.V. 保留所有权利。
The self-assembly of amphiphilic molecules into fibrous structures has been the subject of numerous studies over past decades due to various current and promising technical applications. Although very different in their head group chemistry many natural as well as synthetic amphiphilic compounds derived from carbohydrates, carbocyanine dyes, or amino acids tend to form fibrous structures by molecular self-assembly in water predominantly twisted ribbons or tubes. Often a transition between these assembly structures is observed, which is a phenomenon already theoretically approached by Wolfgang Helfrich and still focus point in current research. With the development of suitable sample preparation and electron optical imaging techniques, cryogenic transmission electron microscopy (cryo-TEM) in combination with three-dimensional (3D) reconstruction techniques has become a particular popular direct characterization technique for supramolecular assemblies in general. Here we review the recent progress in deriving precise structural information from cryo-TEM data of particularly fibrous structures preferably in three dimensions. (c) 2014 Elsevier B.V. All rights reserved.