A saturable receptor for 32P-inositol-l,4,5-trisphosphate in hepatocytes and neutrophils
A saturable receptor for 32P-inositol-l,4,5-trisphosphate in hepatocytes and neutrophils
复制标题
肝细胞和中性粒细胞中 32P-肌醇-1,4,5-三磷酸的可饱和受体
作者：
A. Spät;P. Bradford;J. S. Mckinney;R. Rubin;J. Putney
神经递质、激素和生长因子的几种受体在激活时会加速聚磷酸肌醇的磷酸二酯分解1，2。该反应的可溶性产物之一，肌醇-1，4，5-三磷酸（Ins（1，4，5）P3）被认为充当第二信使，其发出从细胞内储存释放Ca 2+的信号3。为了支持这一假设，几项研究表明，Ins（1，4，5）P3从渗透性细胞4 -6和微粒体7 -9释放螯合的Ca 2+。基于肌醇磷酸对Ca 2+释放活性的某些结构要求，已经假定Ins（1，4，5）P3通过结合特异性细胞内受体（可能在内质网的组分上）起作用10。本文报道了32 P-Ins（1，4，5）P3与透化豚鼠肝细胞和兔中性粒细胞中的一个特异性饱和位点结合，该结合位点的性质表明它是Ins（1，4，5）P3的生理受体。
Several receptors for neurotransmitters, hormones and growth factors cause accelerated phosphodiesteratic breakdown of poly-phosphoinositides when activated1,2. One of the soluble products of this reaction, inositol-1,4,5-trisphosphate (Ins(l,4,5)P3) is thought to act as a second messenger signalling the release of Ca2+ from intracellular stores3. In support of this hypothesis, several studies have shown that Ins(l,4,5)P3 releases sequestered Ca2+ from permeable cells4–6 and microsomes7–9. On the basis of certain structural requirements for Ca2+-releasing activity by inositol phosphates, it has been postulated that Ins(l,4,5)P3 acts by binding to a specific intracellular receptor, probably on a component of the endoplasmic reticulum10. Here we report that 32P-Ins(l,4,5)P3 binds to a specific saturable site in permeabilized guinea pig hepatocytes and rabbit neutrophils, and that the properties of this binding site suggest that it is the physiological receptor for Ins(l,4,5)P3.
皂苷通透的豚鼠肝细胞中的钙池。
DOI：
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发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Burgess,GM;McKinney,JS;Fabiato,A;Leslie,BA;PutneyJr,JW
通讯作者：
PutneyJr,JW