Polyunsaturated Fatty Acids Attenuate Diet Induced Obesity and Insulin Resistance, Modulating Mitochondrial Respiratory Uncoupling in Rat Skeletal Muscle.
Polyunsaturated Fatty Acids Attenuate Diet Induced Obesity and Insulin Resistance, Modulating Mitochondrial Respiratory Uncoupling in Rat Skeletal Muscle.
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DOI：
10.1371/journal.pone.0149033
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Mollica MP
中科院分区：
文献类型：
--
作者：
Cavaliere G;Trinchese G;Bergamo P;De Filippo C;Mattace Raso G;Gifuni G;Putti R;Moni BH;Canani RB;Meli R;Mollica MP
ω-3多不饱和脂肪酸（PUFA）是能够减轻胰岛素抵抗的膳食化合物。然而，ω-3PUFAs在骨骼肌中的确切作用却被忽略了。我们假设PUFA调节线粒体功能和效率，将改善营养诱导的肥胖的促炎和促氧化迹象。为达到此目的，给大鼠喂食对照饮食（CD）或含有ω-3 PUFA（FD）或猪油（LD）的等热量高脂肪饮食6周。FD大鼠表现出较低的体重，脂质增加和能量效率相比，LD喂养的动物，表现出较高的能量消耗和O2消耗/CO2生产。与LD相比，FD喂养动物的血脂谱和促炎参数降低。因此，与LD相比，FD大鼠表现出更高的葡萄糖耐量，这是通过改善的葡萄糖和胰岛素耐量试验揭示的，伴随着骨骼肌中胰岛素信号的恢复。PUFAs增加脂质氧化，降低肌膜下线粒体的能量效率，并增加AMPK活化，减少内质网和氧化应激。线粒体呼吸的增加与FD骨骼肌中纤维生成的增加有关，如PGC 1-α和-β的增加所示。我们的数据加强了高膳食ω3-PUFA摄入与骨骼肌线粒体能量效率降低的关联。
Omega (ω)-3 polyunsaturated fatty acids (PUFA) are dietary compounds able to attenuate insulin resistance. Anyway, the precise actions of ω-3PUFAs in skeletal muscle are overlooked. We hypothesized that PUFAs, modulating mitochondrial function and efficiency, would ameliorate pro-inflammatory and pro-oxidant signs of nutritionally induced obesity. To this aim, rats were fed a control diet (CD) or isocaloric high fat diets containing either ω-3 PUFA (FD) or lard (LD) for 6 weeks. FD rats showed lower weight, lipid gain and energy efficiency compared to LD-fed animals, showing higher energy expenditure and O2 consumption/CO2 production. Serum lipid profile and pro-inflammatory parameters in FD-fed animals were reduced compared to LD. Accordingly, FD rats exhibited a higher glucose tolerance revealed by an improved glucose and insulin tolerance tests compared to LD, accompanied by a restoration of insulin signalling in skeletal muscle. PUFAs increased lipid oxidation and reduced energy efficiency in subsarcolemmal mitochondria, and increase AMPK activation, reducing both endoplasmic reticulum and oxidative stress. Increased mitochondrial respiration was related to an increased mitochondriogenesis in FD skeletal muscle, as shown by the increase in PGC1-α and -β. our data strengthened the association of high dietary ω3-PUFA intake with reduced mitochondrial energy efficiency in the skeletal muscle.