Antibiotics-induced modulation of large intestinal microbiota altered aromatic amino acid profile and expression of neurotransmitters in the hypothalamus of piglets
Antibiotics-induced modulation of large intestinal microbiota altered aromatic amino acid profile and expression of neurotransmitters in the hypothalamus of piglets
复制标题
抗生素诱导的大肠微生物群调节改变了仔猪下丘脑的芳香氨基酸谱和神经递质表达
DOI：
10.1111/jnc.14333
复制
发表时间：
2018-08-01
影响因子：
4.7
通讯作者：
Zhu, Wei-Yun
中科院分区：
文献类型：
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作者：
Gao, Kan;Pi, Yu;Zhu, Wei-Yun
肠道微生物群介导的脑功能调节的证据已从使用无菌啮齿动物或具有口腔寄生虫诱导的微生物群耗竭的动物的研究中得到广泛认可。由于大肠比小肠中含有更多数量和更多样化的微生物，因此大肠微生物群可能在脑功能的调节中发挥关键作用。在这项研究中，一种大肠微生物群靶向策略被用来研究大肠微生物群对脑功能的影响。12头在回肠末端安装T型插管的仔猪（12.0 ± 0.28kg）饲喂标准饲料，并随机分为两组（n=6），分别进行生理盐水或抗生素的回肠输注。输注25天后，分析回肠和粪便微生物群、血清氨基酸和神经递质以及下丘脑转录组学。虽然抗生素输注没有改变近端回肠微生物组成，但它显著改变了粪便微生物组成，并增加了芳香族氨基酸（AAAs）代谢（p
The evidence of gut microbiota-mediated modulation of brain function has been widely recognized from studies using germ-free rodents or animals with oral antibiotic-induced microbiota depletion. Since the large intestine harbors greater numbers and more diverse of microbes than in the small intestine, large intestinal microbiota may play a crucial role in the modulation of brain function. In this study, a large intestinal microbiota-targeted strategy was used to investigate the impact of large intestinal microbiota on brain function. Twelve piglets (12.080.28kg) fitted with a T-cannula at the distal ileum were fed a standard diet and randomly assigned to two groups (n=6) for ileal infusion of either saline or antibiotics. After 25days of infusion, ileal and fecal microbiota, serum amino acids and neurotransmitters, and hypothalamic transcriptomics were analyzed. While the antibiotic infusion did not change the proximal ileal microbial composition, it markedly altered the fecal microbial composition and increased aromatic amino acid (AAAs) metabolism (p