Determination of plasma catecholamines by high performance liquid chromatography with electrochemical detection: comparison with a radioenzymatic method.
Determination of plasma catecholamines by high performance liquid chromatography with electrochemical detection: comparison with a radioenzymatic method.
复制标题
采用电化学检测的高效液相色谱法测定血浆儿茶酚胺：与放射性酶法的比较。
DOI：
10.1016/0024-3205(79)90384-9
复制
发表时间：
1979
期刊：
影响因子：
6.1
通讯作者：
T. Kahan
中科院分区：
文献类型：
--
作者：
P. Hjemdahl;M. Daleskog;T. Kahan
高效液相色谱电化学检测法测定血浆中的儿茶酚胺已被比较的放射酶法。采用色谱法，以0.2-2 ml血浆为样品，以内标物为内标，高精度测定血浆去甲肾上腺素、肾上腺素和多巴胺。放射酶法仅需3 × 50 μl血浆。在一组9个血浆样本中，通过两种方法测定的去甲肾上腺素和肾上腺素浓度的比较显示，两种方法之间具有良好的一致性（分别为r=0.993和0.994）。讨论了这两种方法的优缺点。
High performance liquid chromatography with electrochemical detection has been compared to a radioenzymatic method for the determination of plasma catecholamines. With the use of an internal standard highly accurate determinations of plasma noradrenaline, adrenaline and dopamine were performed on 0.2–2 ml plasma with the chromatographic method. The radioenzymatic method required only 3 × 50 μl plasma. A comparison of noradrenaline and adrenaline concentrations measured by the two methods in a set of nine plasma samples showed an excellent agreement between the methods (r=0.993 and 0.994, respectively). Advantages and disadvantages with the two methods are discussed.