Vernalization-Mediated Epigenetic Silencing by a Long Intronic Noncoding RNA
Vernalization-Mediated Epigenetic Silencing by a Long Intronic Noncoding RNA
复制标题
DOI：
10.1126/science.1197349
复制
发表时间：
2011-01-07
期刊：
影响因子：
56.9
通讯作者：
Sung, Sibum
中科院分区：
文献类型：
--
作者：
Heo, Jae Bok;Sung, Sibum
春化是一种环境诱导的表观遗传开关，在这种开关中，冬季寒冷触发了花抑制因子的表观遗传沉默，从而提供了春季开花的能力。在拟南芥中，冬季寒冷触发花抑制因子开花位点C （FLC）染色质上三甲基化组蛋白H3 Lys（27）的富集，并导致FLC的表观遗传稳定抑制。这种表观遗传变化是由进化上保守的抑制复合体多梳抑制复合体2 （PRC2）介导的。在这里，我们证明了一个长内含子非编码RNA[称为冷辅助内含子非编码RNA (COLDAIR)]是春化介导的FLC表观遗传抑制所必需的。COLDAIR物理上与PRC2的一个组分结合，并将PRC2靶向FLC。我们的研究结果表明COLDAIR是通过与PRC2相互作用在FLC上建立稳定的抑制染色质所必需的。
Vernalization is an environmentally-induced epigenetic switch in which winter cold triggers epigenetic silencing of floral repressors and thus provides competence to flower in spring. In Arabidopsis, winter cold triggers enrichment of tri-methylated histone H3 Lys(27) at chromatin of the floral repressor, FLOWERING LOCUS C (FLC), and results in epigenetically stable repression of FLC. This epigenetic change is mediated by an evolutionarily conserved repressive complex, polycomb repressive complex 2 (PRC2). Here, we show that a long intronic noncoding RNA [termed COLD ASSISTED INTRONIC NONCODING RNA (COLDAIR)] is required for the vernalization-mediated epigenetic repression of FLC. COLDAIR physically associates with a component of PRC2 and targets PRC2 to FLC. Our results show that COLDAIR is required for establishing stable repressive chromatin at FLC through its interaction with PRC2.