Enantioselective changes in oxidative stress and toxin release in Microcystis aeruginosa exposed to chiral herbicide diclofop acid.
Enantioselective changes in oxidative stress and toxin release in Microcystis aeruginosa exposed to chiral herbicide diclofop acid.
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DOI：
10.1016/j.aquatox.2013.10.023
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发表时间：
2014
影响因子：
4.5
通讯作者：
Jing Ye;Ying Zhang;Shengwen Chen;Chaonan Liu;Yongqiang Zhu;Weiping Liu
中科院分区：
文献类型：
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作者：
Jing Ye;Ying Zhang;Shengwen Chen;Chaonan Liu;Yongqiang Zhu;Weiping Liu
研究了手性除草剂双氯草酸对铜绿微囊藻的氧化应激和毒素释放。外消旋双氯草酸、r -双氯草酸和s -双氯草酸诱导活性氧（ROS）生成，增加丙二醛（MDA）浓度，增强超氧化物歧化酶（SOD）活性，促进毒素释放。不同程度的铜绿。MDA浓度升高，SOD活性升高。暴露于双氯草酸后，铜绿杆菌比暴露于theR-和s -对映体的蓝藻发生得更快。此外，还观察了对映体的对映选择性毒性。ther -对映体引发更多的ROS生成，更高的SOD活性和更多的毒素合成和释放。铜绿素细胞比s -对映体双氯草酸及其sr -对映体可通过脂质过氧化和自由基氧化破坏跨膜质子梯度，而s -对映体则不具有这种作用。r -二氯草酸抑制真菌生长。铜绿脓毒苷在早期阶段，但最终诱导更大的毒素释放，这对水柱有有害的影响。这些结果表明，需要更全面的研究来确定对映体的环境安全性，手性农药的应用需要更直接的监督和培训。此外，水生系统中手性污染物的生命周期分析有助于手性农药的环境评价。
Enantioselective oxidative stress and toxin release fromMicrocystis aeruginosaafter exposure to the chiral herbicide diclofop acid were investigated.Racemicdiclofop acid,R-diclofop acid andS-diclofop acid induced reactive oxygen species (ROS) generation, increased the concentration of malondialdehyde (MDA), enhanced the activity of superoxide dismutase (SOD) and triggered toxin release inM. aeruginosato varying degrees. The increase in MDA concentration and SOD activity inM. aeruginosaoccurred sooner after exposure to diclofop acid than when the cyanobacteria was exposed to either theR- and theS-enantiomer. In addition, enantioselective toxicity of the enantiomers was observed. TheR-enantiomer trigged more ROS generation, more SOD activity and more toxin synthesis and release inM. aeruginosacells than theS-enantiomer. Diclofop acid and itsR-enantiomer may collapse the transmembrane proton gradient and destroy the cell membrane through lipid peroxidation and free radical oxidation, whereas theS-enantiomer did not demonstrate such action.R-diclofop acid inhibits the growth ofM. aeruginosain the early stage, but ultimately induced greater toxin release, which has a deleterious effect on the water column. These results indicate that more comprehensive study is needed to determine the environmental safety of the enantiomers, and application of chiral pesticides requires more direct supervision and training. Additionally, lifecycle analysis of chiral pollutants in aquatic system needs more attention to aide in the environmental assessment of chiral pesticides.