Molecular diagnosis of human enteroviruses by phylogeny-based classification by use of the VP4 sequence
Molecular diagnosis of human enteroviruses by phylogeny-based classification by use of the VP4 sequence
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DOI：
10.1086/339298
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发表时间：
2002-03-15
影响因子：
6.4
通讯作者：
Takeda, N
中科院分区：
文献类型：
--
作者：
Ishiko, H;Shimada, Y;Takeda, N
人类肠道病毒（EV）是各种急性和慢性疾病的主要原因。通常进行病毒分离和中和试验以鉴定致病病毒，但这些试验是劳动密集型的，耗时的，有时需要从其中分离出某些病毒的乳鼠。本研究探讨了一种快速可靠的基于逆转录聚合酶链反应和系统发育分析的方法。基于66个原型的VP 4序列，通过相邻连接构建的系统发育树将所有人类EV分为5个不同的簇。这些簇与5个新指定的物种-人EV A-D和脊髓灰质炎病毒密切对应。获得了26个血清型89株分离株的VP 4基因序列。分析了30年以上66个原型菌株的遗传多样性。每个分离株与其各自的原型菌株一起形成单系群沿着，允许血清型鉴定（E-8除外）。基于VP 4的分类似乎是EV分子流行病学研究的有效工具。
Human enteroviruses (EVs) are the major cause of a variety of acute and chronic illnesses. Virus isolation and neutralization tests are usually done to identify the causative virus, but these tests are labor intensive, time consuming, and sometimes require suckling mice from which certain viruses have been isolated. This study investigated a rapid and reliable method based on reverse-transcription polymerase chain reaction and phylogenetic analysis. The phylogenetic tree constructed by neighbor-joining on the basis of the VP4 sequence from 66 prototypes grouped all human EVs into 5 distinct clusters. These clusters correspond closely to the 5 newly designated species-human EV A-D and poliovirus. The VP4 sequences of 89 isolates from 26 serotypes obtained over. 30 years plus those of 66 prototype strains were analyzed. Each isolate formed a monophyletic cluster along with its respective prototype strain, allowing for serotype identification (with the exception of E-8). VP4-based classification appears to be an effective tool for the molecular epidemiology study of EVs.