Ultrabright organic dots with aggregation-induced emission characteristics for cell tracking
Ultrabright organic dots with aggregation-induced emission characteristics for cell tracking
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DOI：
10.1016/j.biomaterials.2014.06.023
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发表时间：
2014-10-01
期刊：
影响因子：
14
通讯作者：
Liu, Bin
中科院分区：
文献类型：
--
作者：
Feng, Guangxue;Tay, Chor Yong;Liu, Bin
非侵入性荧光细胞跟踪提供了关于活跃迁移细胞的生理位移和易位的关键信息，这加深了我们对生物医学工程，肿瘤学研究，干细胞移植和治疗的理解。基于非病毒荧光蛋白转染的细胞示踪已被广泛使用，但存在与细胞类型依赖性表达、滞后读出、免疫原性和诱变相关的问题。因此，需要替代的细胞跟踪方法以在长时间内获得可靠、稳定和有效的标记。在这项工作中，我们成功地开发了具有聚集诱导发射（AIE点）的超亮有机点，并展示了它们用于细胞成像和细胞跟踪的能力。AIE量子点具有高荧光性、超光稳定性、优异的细胞滞留性和生物相容性。与常用的pMAX-GFP质粒标记方法相比，有机AIE点在所有测试的人类细胞系上显示出优异的细胞标记和优越的上级示踪性能，这为基于细胞的免疫治疗和其他相关生物学研究开辟了新的机会。(C)2014爱思唯尔有限公司版权所有。
Noninvasive fluorescence cell tracking provides critical information on the physiological displacement and translocation of actively migrating cells, which deepens our understanding of biomedical engineering, oncological research, stem cell transplantation and therapies. Non-viral fluorescent protein transfection based cell tracing has been widely used but with issues related to cell type-dependent expression, lagged readout, immunogenicity and mutagenesis. Alternative cell tracking methods are therefore desired to attain reliable, stable, and efficient labeling over a long time. In this work, we have successfully developed ultra-bright organic dots with aggregation-induced emission (AIE dots) and demonstrated their capabilities for cellular imaging and cell tracking. The AIE dots possess high fluorescence, super photostability, and excellent cellular retention and biocompatibility. As compared to commonly used pMAX-GFP plasmid labeling approach, the organic AIE dots showed excellent cell labeling on all tested human cell lines and superior tracing performance, which opens up new opportunities in the cell-based immunotherapies and other related biological researches. (C) 2014 Elsevier Ltd. All rights reserved.