cDNA cloning of a new low-density lipoprotein receptor-related protein and mapping of its gene (LRP3) to chromosome bands 19q12-q13.2
cDNA cloning of a new low-density lipoprotein receptor-related protein and mapping of its gene (LRP3) to chromosome bands 19q12-q13.2
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DOI：
10.1006/geno.1998.5339
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发表时间：
1998-07-01
期刊：
影响因子：
4.4
通讯作者：
Yamamoto, TT
中科院分区：
文献类型：
--
作者：
Ishii, H;Kim, DH;Yamamoto, TT
我们在此报告的cDNA克隆的低密度脂蛋白受体（LDLR）家族的一个新成员称为LDLR相关蛋白3（LRP 3）。人和大鼠LRP 3 cDNA编码具有以下区域的770个氨基酸的1型膜蛋白：推定的信号序列、具有RGD序列的两个富含异亮氨酸/亮氨酸/缬氨酸的区域、两个配体结合重复区域、推定的跨膜区域和具有基于酪氨酸的内化信号的富含脯氨酸的胞质区域。人类染色体荧光原位杂交显示，人类LRP 3基因定位于染色体带19 q12-q13.2。LRP 3基因在人体组织中广泛表达，在骨骼肌和卵巢中表达最高。尽管存在配体结合重复序列，但转染LRP 3的CHO细胞不结合β-迁移极低密度脂蛋白或受体相关蛋白。(C)北京：科学出版社.
We report herein the cDNA cloning of a novel member of the low-density lipoprotein receptor (LDLR) family termed LDLR-related protein 3 (LRP3). Human and rat LRP3 cDNAs encode a 770-amino-acid type 1 membrane protein with the following regions: a putative signal sequence, two isoleucine/leucine/valine-rich regions with an RGD sequence, two ligand-binding repeat regions, a putative transmembrane region, and a proline-rich cytoplasmic region with a tyrosine-based internalization signal. Fluorescence in situ hybridization of human chromosomes revealed that the human LRP3 gene mapped to chromosome bands 19q12-q13.2. LRP3 transcript was detected in a wide range of human tissues, with the highest expression in skeletal muscle and ovary. Despite the presence of the ligand-binding repeats, CHO cells transfected with LRP3 did not bind beta-migrating very-low-density lipoprotein or receptor-associated protein. (C) 1998 Academic Press.