He-Ne laser preillumination improves the resistance of tall fescue (Festuca arundinacea Schreb.) seedlings to high saline conditions
He-Ne laser preillumination improves the resistance of tall fescue (Festuca arundinacea Schreb.) seedlings to high saline conditions
复制标题
氦氖激光预照射提高高羊茅（Festuca arundinacea Schreb.）幼苗对高盐条件的抵抗力
DOI：
10.1007/s00709-014-0748-3
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发表时间：
2015-07-01
期刊：
影响因子：
2.9
通讯作者：
Han, Rong
中科院分区：
文献类型：
--
作者：
Gao, Li-Mei;Li, Yong-Feng;Han, Rong
本文探讨了He-Ne激光预照射对高羊茅幼苗耐高盐胁迫的保护作用及其生理生化机制。结果表明，盐胁迫显著降低了植物生长、株高、生物量、叶片发育、抗坏血酸（阿萨）和谷胱甘肽（GSH）含量、过氧化氢酶（CAT）和谷胱甘肽还原酶（GR）等抗氧化酶的活性和基因表达水平，提高了过氧化氢（H2 O2）含量、超氧自由基（O2·−）产生速率、膜脂过氧化、相对电解质渗漏，酶活性，超氧化物歧化酶（SOD），抗坏血酸过氧化物酶（APX）和过氧化物酶（POD）的基因表达水平，与对照相比。然而，He-Ne激光预照射显着逆转盐胁迫诱导的植物生长迟缓，生物量损失，叶片发育衰退。He-Ne激光可部分恢复或进一步提高盐胁迫下的生理生化指标。盐胁迫对光敏色素B的转录活性无明显影响，而He-Ne激光则显著提高了光敏色素B的转录水平。用白色荧光灯（W）、相同波长的红光（RL）或RL、然后用远红光（FRL）预照射均不能缓解盐胁迫对植物生长和抗氧化酶活性的抑制作用，这表明He-Ne激光提高植物耐盐性的作用很可能是通过激光预照射诱导光敏色素B的转录活性，而不是RL。FRL或其他光源。此外，我们还利用硝普钠（SNP）作为NO供体，在相同条件下对高羊茅幼苗进行预处理，进一步评价了He-Ne激光和NO在提高高羊茅抗盐性方面的生理效应差异。以上结果表明，He-Ne激光预照射通过清除自由基、诱导植物抗氧化系统相关基因的转录活性，减轻氧化损伤，从而提高高羊茅幼苗对盐胁迫的耐受性，其中光敏色素B的转录水平可能与He-Ne激光预照射诱导的这些过程有关。He-Ne激光的这种积极的生理化学效应似乎比NO分子更有效。
In this paper, we explored the protective effect and physiochemical mechanism of He-Ne laser preillumination in enhancement of tall fescue seedlings tolerance to high salt stress. The results showed that salt stress greatly reduced plant growth, plant height, biomass, leaf development, ascorbate acid (AsA) and glutathione (GSH) concentration, the enzymatic activities, and gene expression levels of antioxidant enzymes such as catalase (CAT) and glutathione reductase (GR) and enhanced hydrogen peroxide (H2O2) content, superoxide radical (O2·−) generation rates, membrane lipid peroxidation, relative electrolyte leakage, the enzymatic activities, and gene expression levels of superoxide dismutase (SOD), ascorbate peroxidase (APX), and peroxidase (POD), compared with controls. However, He-Ne laser preillumination significantly reversed plant growth retardation, biomass loss, and leaves development decay induced by salt stress. And the values of the physiochemical parameters observed in salt-stressed plants were partially reverted or further increased by He-Ne laser. Salt stress had no obvious effect on the transcriptional activity of phytochromeB, whereas He-Ne laser markedly enhanced its transcriptional level. Preillumination with white fluorescent lamps (W), red light (RL) of the same wavelength, or RL, then far-red light (FRL) had not alleviated the inhibitory effect of salt stress on plant growth and antioxidant enzymes activities, suggesting that the effect of He-Ne laser on improved salt tolerance was most likely attributed to the induction of phytochromeB transcription activities by the laser preillumination, but not RL, FRL or other light sources. In addition, we also utilized sodium nitroprusside (SNP) as NO donor to pre-treat tall fescue seedlings at the same conditions, and further evaluated the differences of physiological effects between He-Ne laser and NO in increasing salt resistance of tall fescue. Taken together, our data illustrated that He-Ne laser preillumination contributed to conferring an increased tolerance to salt stress in tall fescue seedlings due to alleviating oxidative damage through scavenging free radicals and inducing transcriptional activities of some genes involved in plant antioxidant system, and the induction of phytochromeB transcriptional level by He-Ne laser was probably correlated with these processes. Moreover, this positive physiochemical effect seemed more effective with He-Ne laser than NO molecule.