Sugar-regulated control of α-tubulin in maize cell suspension culture
Sugar-regulated control of α-tubulin in maize cell suspension culture
复制标题
DOI：
10.1007/s002990000310
复制
发表时间：
2001-03-01
影响因子：
6.2
通讯作者：
Chourey, PS
中科院分区：
文献类型：
--
作者：
Datta, R;Chourey, PS
本文报道的数据表明，细胞骨架中的微管蛋白亚基α -微管蛋白受糖调节。在EMS玉米细胞悬浮培养基中进行48小时的糖缺失处理，导致α -微管蛋白mRNA和蛋白水平显著降低。这种反应是可逆的；可代谢糖的添加导致α -微管蛋白转录物水平的迅速增加。α -微管蛋白的水平也增加了，尽管是逐渐增加的。令人惊讶的是，磷酸盐消耗和磷酸盐添加对α -微管蛋白表达的影响很小或没有影响。此外，数据显示糖调节α -微管蛋白和细胞壁转化酶的共同调节。我们提出微管可能是糖信号通路的主要组成部分，该通路受细胞碳代谢等因素的调节。
The data reported here shows that alpha -tubulin, the subunit of microtubulin in the cytoskeleton, is regulated by sugars. A 48-h sugar-depletion treatment in EMS maize cell suspension culture medium led to a marked reduction in the levels of alpha -tubulin mRNA and protein. This response was reversible; the addition of metabolizable sugars led to a rapid increase in the levels of the alpha -tubulin transcript. Levels of alpha -tubulin protein also increased, albeit gradually. Surprisingly, there was little or no effect of phosphate depletion and phosphate addition on alpha -tubulin expression. Furthermore, the data show that sugars modulate a co-regulation of alpha -tubulin and cell-wall invertase. We propose that microtubules might be a major component of a sugar-signaling pathway that is regulated by, among other factors, cellular carbon metabolism.