IL-22 mRNA in peripheral blood mononuclear cells from allergic rhinitic and asthmatic pediatric patients
IL-22 mRNA in peripheral blood mononuclear cells from allergic rhinitic and asthmatic pediatric patients
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DOI：
10.1111/j.1399-3038.2010.01116.x
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发表时间：
2011-06-01
影响因子：
4.4
通讯作者：
Santoni, Giorgio
中科院分区：
文献类型：
--
作者：
Farfariello, Valerio;Amantini, Consuelo;Santoni, Giorgio
辅助性T（T（H））-17淋巴细胞表达多种调节性细胞因子，包括IL-17 A和IL-22，但目前还没有关于过敏性哮喘和鼻炎（AR）患儿外周血单个核细胞（PBMC）表达这些细胞因子的临床资料。本研究的目的是探讨血清过敏性反应、AR和哮喘儿童中IL-22和IL-17 A mRNA与临床参数之间的可能关系。这项研究在花粉季节进行，包括18名健康儿童和18名过敏儿童（n = 9名哮喘儿童和n = 9名鼻病儿童）。进行血清总IgE和特异性IgE、嗜酸性粒细胞计数和皮肤点刺试验;此外，通过定量实时PCR检测健康和过敏受试者PBMC中IL-22和IL-17 A mRNA水平。尽管IL-17 A细胞因子的mRNA的情况下，IL-22的表达被发现在PBMC从哮喘患者，与增加的IL-22的mRNA水平在慢性重度哮喘患者的中度哮喘。哮喘患儿IL-22 mRNA水平与血清总IgE水平呈正相关。此外，与健康个体相比，在AR和无症状血清过敏性儿童中检测到更高的IL-22和IL-17 A mRNA水平，并且证实了IL-22和IL-17 A mRNA与血清总IgE水平之间的相关性。此外，维甲酸相关孤儿受体C（T（H）17转录因子）的mRNA水平在AR中增加，但在哮喘患者中不增加。本研究首次证明IL-22 mRNA在慢性重症哮喘和AR儿童中可能有表达。在无症状的单致敏血清过敏性儿童中IL-22和IL-17 A mRNA的表达表明这些细胞因子在这些过敏性疾病的发展中所涉及的早期事件中的作用。
P>T helper (T(H))-17 lymphocytes are characterized by the expression of many regulatory cytokines, including IL-17A and IL-22, but at present no clinical data are available on the expression of these cytokines in peripheral blood mononuclear cells (PBMC) from allergic asthmatic and rhinitic (AR) children. The aim of this study was to investigate a possible relationship between IL-22 and IL-17A mRNAs and clinical parameters in seroatopic, AR, and asthmatic children. The study, conduced during the pollen season, included 18 healthy and 18 allergic (n = 9 asthmatic and n = 9 rhinitic) children. Serum total and specific IgE, eosinophil count, and skin prick test were performed; in addition, IL-22 and IL-17A mRNA levels were detected in PBMC from healthy and allergic subjects by quantitative real-time PCR. Despite the absence of the mRNA for the IL-17A cytokine, IL-22 expression was found in PBMC from asthmatic patients, with increased IL-22 mRNA levels in patients with chronic severe respect to those with moderate asthma. A positive correlation between IL-22 mRNA and serum total IgE levels was found in asthmatic children. In addition, higher IL-22 and IL-17A mRNA levels were detected in both AR and asymptomatic seroatopic children, compared to healthy individuals, and a correlation between IL-22 and IL17A mRNA and serum total IgE levels was demonstrated. Moreover, the mRNA level of retinoic acid-related orphan receptor C, the T(H)17 transcription factor, was found to be increased in AR but not in asthmatic patients. This study provides the first evidence that IL-22 mRNA might be expressed in chronic severe asthmatic and AR children. The expression of IL-22 and IL-17A mRNAs in asymptomatic monosensitized seroatopic children suggests a role of these cytokines in the early events involved in the development of these allergic diseases.