Cell division in higher plants: a cdc2 gene, its 34-kDa product, and histone H1 kinase activity in pea.
Cell division in higher plants: a cdc2 gene, its 34-kDa product, and histone H1 kinase activity in pea.
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高等植物中的细胞分裂：豌豆中的 cdc2 基因、其 34-kDa 产物和组蛋白 H1 激酶活性。
DOI：
10.1073/pnas.87.14.5397
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发表时间：
1990
影响因子：
11.1
通讯作者：
Thomas Jacobs
中科院分区：
文献类型：
--
作者：
H. Feiler;Thomas Jacobs
酵母和动物细胞的有丝分裂细胞周期受cdc2基因及其产物p34蛋白激酶以及MPF或组蛋白H1激酶复合体的其他成分的调节。我们提出的证据表明，cdc2、p34和组蛋白H1激酶也存在于高等植物中。被调查的10种植物的蛋白质提取物显示出一种34 kDa的成分，可以被一种针对分裂酵母p34的进化保守表位的单抗识别。豌豆有丝分裂组织的非变性蛋白提取液经凝胶过滤、变性条件下电泳法分离、免疫印迹。在低分子质量组分和高分子质量组分中都有明显的P34交叉反应物质，这表明豌豆P34既以单体形式存在，也作为高分子质量络合物的一部分存在。组蛋白H1激酶活性主要存在于较高分子质量组分中，即豌豆p34的磷酸化程度最低的组分。我们还报道了通过聚合酶链式反应克隆了cdc2的豌豆同源物。DNA序列分析显示，到目前为止，在所有分析的CDC2基因产品中都发现了标记“PSTAIR”序列基序的完美保守。
The mitotic cell cycle of yeast and animal cells is regulated by the cdc2 gene and its product, the p34 protein kinase, and by other components of the MPF or histone H1 kinase complex. We present evidence that cdc2, p34, and a histone H1 kinase also exist in higher plants. Protein extracts from 10 plant species surveyed display a 34-kDa component recognized by a monoclonal antibody directed against an evolutionarily conserved epitope of fission yeast p34. Nondenatured protein extracts of mitotic Pisum sativum (garden pea) tissues were fractionated by gel filtration, electrophoretically separated under denaturing conditions, and immunoblotted. p34 crossreactive material was apparent in both low and high molecular mass fractions, indicating that pea p34 occurs as both a monomer and as part of a high molecular mass complex. Histone H1 kinase activity was found predominantly in the higher molecular mass fractions, those to which the least phosphorylated form of pea p34 was confined. We also report the cloning of the pea homologue of cdc2 by polymerase chain reaction. DNA sequence analysis reveals perfect conservation of the hallmark "PSTAIR" sequence motif found in all cdc2 gene products analyzed to date.