On-chip handling of solutions and electrochemiluminescence detection of amino acids
On-chip handling of solutions and electrochemiluminescence detection of amino acids
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DOI：
10.1016/j.snb.2006.06.030
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发表时间：
2007-03
影响因子：
8.4
通讯作者：
H. Hosono;W. Satoh;J. Fukuda;H. Suzuki
中科院分区：
文献类型：
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作者：
H. Hosono;W. Satoh;J. Fukuda;H. Suzuki
在微流体传输、溶液混合和ECL生成之后，通过电化学发光（ECL）进行氨基酸的芯片上测定。所制造的系统由具有流动通道结构的聚二甲基硅氧烷（PDMS）衬底和形成在玻璃衬底上的三电极系统组成，所述三电极系统导致微流体传输和ECL。氨基酸溶液和含有三（2，2 ′-联吡啶）钌（II）（Ru（bpy）32+）的试剂溶液通过向沿流动通道沿着形成的金工作电极施加负电位而通过电润湿进行输送。然后使用在电极之间形成的用于输送的附加金工作电极在混合通道中混合两种溶液。通过向在混合通道中形成的铂工作电极施加电势来产生ECL。检测了L-脯氨酸、L-赖氨酸、L-亮氨酸、L-缬氨酸和L-组氨酸的ECL。观察到清晰的ECL，其强度依赖于氨基酸的浓度。通过混合溶液增加pH值并在芯片上产生氧化形式Ru（bpy）33+，可以提高检测灵敏度。l-脯氨酸显示出最强的ECL，并且可以检测到nM级的浓度。
On-chip determination of amino acids was conducted by electrochemiluminescence (ECL) following microfluidic transport, mixing of solutions, and generation of ECL. The fabricated system consisted of a polydimethylsilixane (PDMS) substrate with a flow channel structure and three-electrode systems formed on a glass substrate that resulted in the microfluidic transport and the ECL. An amino acid solution and a reagent solution containing tris(2,2′-bipyridyl)ruthenium(II) (Ru(bpy)32+) were transported by electrowetting by applying a negative potential to gold working electrodes formed along the flow channels. The two solutions were then mixed in the mixing channel using an additional gold working electrode formed between the electrodes for transport. ECL was generated by applying a potential to a platinum working electrode formed in the mixing channel. The ECL from l-proline, l-lysine, l-leucine, l-valine, and l-histidine was examined. Clear ECL was observed, and its intensity depended on the concentration of the amino acids. By increasing the pH by mixing solutions and generating the oxidized form, Ru(bpy)33+, on the chip, the detection sensitivity could be improved. l-proline showed the strongest ECL, and concentrations of the nM order could be detected.