MAMMALIAN OOCYTES EXHIBIT SPECIFIC RECOGNITION OF THE RGD (ARG-GLY-ASP) TRIPEPTIDE AND EXPRESS OOLEMMAL INTEGRINS
MAMMALIAN OOCYTES EXHIBIT SPECIFIC RECOGNITION OF THE RGD (ARG-GLY-ASP) TRIPEPTIDE AND EXPRESS OOLEMMAL INTEGRINS
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DOI：
10.1002/mrd.1080360212
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发表时间：
1993-10-01
影响因子：
2.5
通讯作者：
BRONSON, RA
中科院分区：
文献类型：
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作者：
FUSI, FM;VIGNALI, M;BRONSON, RA
整合素是一个细胞粘附受体家族，参与许多细胞-细胞和细胞-基质相互作用。这些异源二聚体受体中的一些，例如α 5 β 1和α v β 1，特异性识别其配体内的氨基酸序列Arg-Gly-Asp（RGD）。RGD序列存在于纤连蛋白、玻连蛋白和其他细胞外基质蛋白中。我们的研究结果表明，从人类和其他几种哺乳动物的卵细胞膜含有能够结合到RGD配体的受体，整合素亚基的卵母细胞表达。利用四种不同的技术来鉴定哺乳动物卵上功能性整合素的存在。在所有受试物种的无透明带卵表面检测到RGD结合受体。用含有RGD的Peptide-2000包被的Covaspheres与卵结合并形成ROBLET。玫瑰花结被肽-2000和GRGDTP（一种可溶性RGD肽）竞争性抑制，但RGES不抑制。使用针对整合素亚基胞质尾部的多克隆抗体的ELISA在叙利亚仓鼠卵的洗涤剂提取物中鉴定了整合素亚基α 5、β 1和α v，但未鉴定β 3。斑点印迹证实了仓鼠卵裂解物中存在α病毒。最后，整合素亚基α 2，α 5，α 6，但不是α 4，检测到的表面上的透明带的鸡蛋从人类和叙利亚仓鼠。用α 2、α 5和α 6特异性单克隆抗体包被的免疫球与卵结合并形成了卵细胞。从包被有抗玻连蛋白受体（α v β 3/β 5）的多克隆抗体的珠粒获得类似的玫瑰花结。这些观察结果表明整合素α 2 β 1、α 5 β 1、α 6 β 1和一种或多种含有α v的整合素由卵母细胞表达。我们认为这些整合素可能在受精和着床过程中发挥重要作用。 (C)1993 Wiley-Liss，Inc.
Integrins are a family of cell adhesion receptors involved in many cell-cell and cell-matrix interactions. Some of these heterodimeric receptors, such as alpha5beta1 and alphavbeta1, specifically recognize the amino acid sequence Arg-Gly-Asp (RGD) within their ligands. The RGD sequence is found in fibronectin, vitronectin, and other extracellular matrix proteins. Our results demonstrate that the oolemmas of eggs from human and several other mammalian species contain receptors capable of binding to RGD ligands, and that integrin subunits are expressed by oocytes. Four distinct techniques were utilized to identify the presence of functional integrins on mammalian eggs. RGD-binding receptors were detected on the surfaces of zona-free eggs from all species tested. Covaspheres coated with PepTide-2000, which contains RGD, bound to the eggs and formed rosettes. Rosetting was competitively inhibited by Peptide-2000 and by GRGDTP, a soluble RGD peptide, but not by RGES. An ELISA using polyclonal antibodies directed against the cytoplasmic tails of the integrin subunits identified the integrin subunits alpha5, beta1, and alphav, but not beta3, in detergent extracts of Syrian hamster eggs. A dot blot confirmed the presence of alphav in hamster egg lysates. Finally, the integrin subunits alpha2, alpha5, alpha6, but not alpha4, were detected on the surfaces of zona-free eggs from human and Syrian hamster. Immunobeads coated with monoclonal antibodies specific for alpha2, alpha5, and alpha6 bound to the eggs and formed rosettes. A similar rosetting was obtained from beads coated with polyclonal antibody against the vitronectin receptor (alphavbeta3/beta5). These observations suggest that the integrins alpha2beta1, alpha5beta1, alpha6beta1, and one or more integrins containing alphav are expressed by oocytes. We propose that these integrins may play important roles in fertilization and implantation. (C) 1993 Wiley-Liss, Inc.