Biosensor Based on Ultrasmall MoS2 Nanoparticles for Electrochemical Detection of H2O2 Released by Cells at the Nanomolar Level
Biosensor Based on Ultrasmall MoS2 Nanoparticles for Electrochemical Detection of H2O2 Released by Cells at the Nanomolar Level
复制标题
基于超小 MoS2 纳米粒子的生物传感器，用于电化学检测细胞释放的纳摩尔水平的 H2O2。
DOI：
10.1021/ac402114c
复制
发表时间：
2013-11-05
影响因子：
7.4
通讯作者：
Li, Meixian
中科院分区：
文献类型：
--
作者：
Wang, Tanyuan;Zhu, Haichuan;Li, Meixian
以二硫化钼为原料，通过超声破碎和梯度离心法制备了粒径小于2 nm的无表面活性剂的单分散二硫化钼纳米粒子。超小的二硫化钼纳米颗粒暴露了大部分的边缘位点，沿着与它们的高表面积，这导致有吸引力的电催化活性还原H2O2。构建了一种基于二硫化钼纳米颗粒的H2O2生物传感器，其真实的检测限低至2.5 nM，线性范围宽达5个数量级。在此基础上，成功地记录了Raw 264.7细胞释放的痕量H2O2，并制备了高效的葡萄糖生物传感器。由于H2O2是许多氧化生物反应的副产物，因此这项工作为MoS2在电化学传感和生物分析领域的应用提供了途径。
Monodispersed surfactant-free MoS2 nanoparticles with sizes of less than 2 nm were prepared from bulk MoS2 by simple ultrasonication and gradient centrifugation. The ultrasmall MoS2 nanoparticles expose a large fraction of edge sites, along with their high surface area, which lead to attractive electrocatalytic activity for reduction of H2O2. An extremely sensitive H2O2 biosensor based on MoS2 nanoparticles with a real determination limit as low as 2.5 nM and wide linear range of 5 orders of magnitude was constructed. On the basis of this biosensor, the trace amount of H2O2 released from Raw 264.7 cells was successfully recorded, and an efficient glucose biosensor was also fabricated. Since H2O2 is a byproduct of many oxidative biological reactions, this work serves as a pathway for the application of MoS2 in the fields of electrochemical sensing and bioanalysis.