Spatiotemporal dynamics of bacterial populations in the anoxic Cariaco Basin
Spatiotemporal dynamics of bacterial populations in the anoxic Cariaco Basin
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DOI：
10.4319/lo.2008.53.1.0037
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发表时间：
2008-01-01
影响因子：
4.5
通讯作者：
Taylor, Gordon T.
中科院分区：
文献类型：
--
作者：
Lin, Xueju;Scranton, Mary I.;Taylor, Gordon T.
使用荧光原位杂交（FISH）和16 S核糖体核糖核酸（rRNA）基因的末端限制性片段长度多态性（T-RFLP），在三个站点对缺氧的Cariaco盆地的细菌种群分布进行了为期2年（5次巡航）的研究。FISH结果表明，ε-蛋白细菌丰度对总原核生物库存的贡献在各航次之间相对恒定，并且该组在氧化还原中占主导地位，平均占DAPI（4 '6-diamidino-2-phenylindole）染色计数的18%。相比之下，β-变形菌的变异性更大。硫酸盐还原菌，α-和γ-变形菌占厌氧微生物群落的次要组成部分。T-RFLP指纹图谱也显示了细菌群落组成的显着时间变化。好氧区、过渡区和缺氧区的细菌群落结构差异最大，但三个不同站点的氧化还原带内也存在显着的水平差异。T-RFLP模式的统计分析揭示了明确的时空模式，细菌群落结构沿着氧化还原梯度之间的网站和随着时间的推移。总原核生物丰度与氧化还原跃层以上的有机碳通量的季节性变化相对应。然而，没有一个单一的测量环境变量（O-2，H2S，S2 O32-，H4 SiO 4，NO3-，PO 43-，微生物生物量，化能自养生产）解释的时间振荡的优势菌群。分析表明，氧化还原梯度，陆地输入，和干扰，由于邻近的沃茨的横向入侵可能会显着影响细菌群落组成的Cariaco盆地。
Distributions of bacterial populations in the anoxic Cariaco Basin were studied over 2 yr (five cruises) at three stations using fluorescence in situ hybridization (FISH) and terminal restriction fragment length polymorphism (T-RFLP) of 16S ribosomal ribonucleic acid (rRNA) genes. FISH results demonstrate that the contribution of epsilon-proteobacterial abundances to total prokaryotic inventories was relatively constant among cruises, and this group was dominant in the redoxcline, accounting for 18% of DAPI (4'6-diamidino-2-phenylindole) - stained counts on average. In contrast, beta-proteobacteria were more variable. Sulfate reducers, alpha-, and gamma-proteobacteria accounted for minor components of the anaerobic microbial communities. T-RFLP fingerprinting also showed significant temporal shifts in bacterial community composition. The greatest variations in bacterial community structure were evident among oxic, transitional, and anoxic zones, but significant horizontal variations were also evident within redoxclines of three different stations. A statistical analysis of T-RFLP patterns revealed clear spatiotemporal patterns in bacterial community structure along the redox gradient among sites and over time. Total prokaryotic abundances corresponded well with seasonal variations in organic carbon fluxes above the redoxcline. However, no single measured environmental variable (O-2, H2S, S2O32-, H4SiO4, NO3-, PO43-, microbial biomass, chemoautotrophic production) explained the temporal oscillations of the dominant bacterial groups. Analyses suggest that redox gradients, terrestrial inputs, and disturbance due to lateral intrusions of adjacent waters could significantly affect bacterial community composition in the Cariaco Basin.