De novo protein design: how do we expand into the universe of possible protein structures?
De novo protein design: how do we expand into the universe of possible protein structures?
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DOI：
10.1016/j.sbi.2015.05.009
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发表时间：
2015-08-01
影响因子：
6.8
通讯作者：
Wood, Christopher W.
中科院分区：
文献类型：
--
作者：
Woolfson, Derek N.;Bartlett, Gail J.;Wood, Christopher W.
蛋白质科学家正在为蛋白质设计和工程的新阶段铺平道路。正在开发的途径和方法可以使蛋白质的设计超越天然蛋白质结构的限制。这种设计全新蛋白质的可能性带来了新的问题：我们构建的是什么？我们如何构建蛋白质结构空间，在那里几乎没有，如果有的话，自然结构来指导我们？产生的蛋白质有什么用途？而且，如果有的话，这种追求能告诉我们天然蛋白质是如何折叠、功能和进化的吗？我们描述了这一新兴领域的完全从头蛋白质设计的起源，它是如何开发的，它可能会导致什么，以及未来的挑战。
Protein scientists are paving the way to a new phase in protein design and engineering. Approaches and methods are being developed that could allow the design of proteins beyond the confines of natural protein structures. This possibility of designing entirely new proteins opens new questions: What do we build? How do we build into protein-structure space where there are few, if any, natural structures to guide us? To what uses can the resulting proteins be put? And, what, if anything, does this pursuit tell us about how natural proteins fold, function and evolve? We describe the origins of this emerging area of fully de novo protein design, how it could be developed, where it might lead, and what challenges lie ahead.