Inhibition of Epstein-Barr virus lytic cycle by (-)-epigallocatechin gallate
Inhibition of Epstein-Barr virus lytic cycle by (-)-epigallocatechin gallate
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DOI：
10.1016/s0006-291x(03)00067-6
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发表时间：
2003-02-21
影响因子：
3.1
通讯作者：
Liu, ST
中科院分区：
文献类型：
--
作者：
Chang, LK;Wei, TT;Liu, ST
（-）-表没食子儿茶素没食子酸酯（Epigallocatechin gallate，EGCG）是一种有效的抗微生物和抗肿瘤化合物，富含于绿色茶中。本研究使用免疫印迹、流式细胞术、微阵列和间接免疫荧光分析显示，在超过50 μ M的浓度下，EGCG抑制EB病毒（EBV）裂解蛋白的表达，包括Rta、Zta和EA-D，但不影响EBNA-1的表达。此外，DNA微阵列和瞬时转染分析表明，EGCG通过抑制立即早期基因的转录来阻断EBV裂解周期，从而抑制EBV裂解级联反应的启动。(C)2003 Elsevier Science（美国）。All rights reserved.
(-)-Epigallocatechin gallate (EGCG), abundant in green tea, is a potent anti-microbial and anti-tumor compound. This investigation used immunoblot, flow cytometry, microarray, and indirect immunofluorescence analyses to show that at concentrations exceeding 50 muM, EGCG inhibits the expression of Epstein-Barr virus (EBV) lytic proteins, including Rta, Zta, and EA-D, but does not affect the expression of EBNA-1. Moreover, DNA microarray and transient transfection analyses demonstrated that EGCG blocks EBV lytic cycle by inhibiting the transcription of immediate-early genes, thus inhibiting the initiation of EBV lytic cascade. (C) 2003 Elsevier Science (USA). All rights reserved.