Proteomic analysis of a twin-arginine translocation-deficient mutant unravel its functions involved in stress adaptation and virulence in fish pathogen Edwardsiella tarda.
Proteomic analysis of a twin-arginine translocation-deficient mutant unravel its functions involved in stress adaptation and virulence in fish pathogen Edwardsiella tarda.
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DOI：
10.1111/1574-6968.12140
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发表时间：
2013-06
影响因子：
2.1
通讯作者：
Yamin Wang;Qiyao Wang;Minjun Yang;Yuanxing Zhang
中科院分区：
文献类型：
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作者：
Yamin Wang;Qiyao Wang;Minjun Yang;Yuanxing Zhang
迟缓爱德华氏菌（Edwardsiellatarda）是一种革兰氏阴性兼性需氧病原菌，可感染鱼类、两栖类和人类等多种宿主。在大肠杆菌中发现了一个重要的双精氨酸易位（达特）基因簇。此前已确认了Tarda。在此，进一步表征了达特系统的遗传结构和在细菌生理适应中的多效性作用。功能分析表明，tatD不是达特输出所必需的，tatE可能是tatA的等位基因。结果表明，达特系统的破坏对E.迟发性，但确实影响真核细胞（例如巨噬细胞J774a）的运动性、血凝、细胞聚集和感染。采用双向凝胶电泳和鸟枪蛋白质定量测序技术对大肠杆菌中的亚细胞蛋白质进行比较蛋白质组学分析，以鉴定大肠杆菌中差异表达的蛋白质。延迟EIB 202与延迟ABCD。结果显示了大量差异表达蛋白（n = 61），阐明了与大肠杆菌毒力和胁迫相关过程相关的达特系统。迟到。
Edwardsiella tarda is a Gram-negative, facultative aerobic pathogen which infects multifarious hosts including fish, amphibians and human beings. A twin-arginine translocation (Tat) gene cluster important for high-salt tolerance in E. tarda was identified previously. Here the genetic structure and pleiotropic roles of the Tat system in physiological adaptation of the bacterium were further characterized. Functional analysis indicated that tatD was not required for Tat export process and tatE might be an allelic gene of tatA in the bacterium. The results showed that disruption in the Tat system did not affect the morphology and biofilm formation in E. tarda, but did affect motility, hemagglutination, cell aggregation and infection of eukaryotic cells (e.g. macrophage J774a). Comparative proteomics analysis of subcellular proteins using two-dimensional gel electrophoresis and a qualitative shotgun protein sequencing method were implemented to identify proteins differentially expressed in E. tarda EIB202 vs. ∆tatABCD. The results revealed a large repertoire of differentially expressed proteins (n = 61), shedding light on the Tat system associated with virulence and stress-associated processes in E. tarda.