Integrative effects based on behavior, physiology and gene expression of tritiated water on zebrafish
Integrative effects based on behavior, physiology and gene expression of tritiated water on zebrafish
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基于氚水对斑马鱼行为、生理和基因表达的综合效应
DOI：
10.1016/j.ecoenv.2021.112770
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发表时间：
2021
影响因子：
6.8
通讯作者：
Tu Yu
中科院分区：
文献类型：
--
作者：
Li Shengri;Zhang Yefeng;Xue Huiyuan;Zhang Qixuan;Chen Na;Wan Jun;Sun Liang;Chen Qiu;Zong Ying;Zhuang Fenghui;Gu Pengcheng;Zhang Anqi;Cui Fengmei;Tu Yu
氚是一种水溶性氢同位素，在衰变过程中释放β射线。在自然界中，氚主要以氚化水（HTO）的形式存在，其主要来源是核电站/加工厂。近几十年来，随着核电工业的发展，有必要评估氚对生物体的影响。在本研究中，受精斑马鱼胚胎用不同浓度的 HTO（3.7 × 103Bq/ml、3.7 × 104Bq/ml、3.7 × 105Bq/ml）处理。经过HTO处理后，斑马鱼胚胎的发育没有明显的形态变化。然而，心率增加，运动活动显着下降。此外，RNA 测序表明 HTO 可以影响基因表达。差异表达基因通过许多生理过程和细胞内信号通路而丰富，包括心脏、心血管和神经系统发育以及细胞色素P450对异生物质的代谢。此外，经HTO处理后，斑马鱼体内甲状腺激素浓度降低，甲状腺激素相关基因表达紊乱。研究结果表明，HTO暴露可能通过生理过程和细胞内信号通路影响斑马鱼的生理和行为，为氚的生态风险评估提供理论依据。
Tritium is a water-soluble hydrogen isotope that releases beta rays during decay. In nature, tritium primarily exists as tritiated water (HTO), and its main source is nuclear power/processing plants. In recent decades, with the development of nuclear power industry, it is necessary to evaluate the impact of tritium on organisms. In this study, fertilized zebrafish embryos are treated with different HTO concentrations (3.7 × 103Bq/ml, 3.7 × 104Bq/ml, 3.7 × 105Bq/ml). After treatment with HTO, the zebrafish embryos developed without evident morphological changes. Nevertheless, the heart rate increased and locomotor activity decreased significantly. In addition, RNA-sequencing shows that HTO can affect gene expressions. The differentially expressed genes are enriched through many physiological processes and intracellular signaling pathways, including cardiac, cardiovascular, and nervous system development and the metabolism of xenobiotics by cytochrome P450. Moreover, the concentrations of thyroid hormones in the zebrafish decrease and the expression of thyroid hormone-related genes is disordered after HTO treatment. Our results suggest that exposure to HTO may affect the physiology and behaviors of zebrafish through physiological processes and intracellular signaling pathways and provide a theoretical basis for ecological risk assessment of tritium.