Region-specific modulation of electrically induced synchronous oscillations in the rat hippocampus and cerebral cortex
Region-specific modulation of electrically induced synchronous oscillations in the rat hippocampus and cerebral cortex
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DOI：
10.1016/j.neures.2005.01.012
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发表时间：
2005-05-01
影响因子：
2.9
通讯作者：
Takada, M
中科院分区：
文献类型：
--
作者：
Kaneda, K;Fujiwara-Tsukamoto, Y;Takada, M
强强直刺激诱导海马CA 1区成熟锥体细胞的同步膜电位振荡（类似于后放电）。为了研究其他脑区的局部网络是否可以独立地产生这样的后放电，我们使用细胞内和细胞外记录研究了大鼠海马CA 1和CA 3区、齿状回（DG）、内嗅皮层（EC）和颞叶皮层（TC）的单个“孤立切片”中后放电的诱导。强强直刺激在海马CA 1和CA 3区锥体细胞以及EC和TC表面主细胞中持续诱发后放电。然而，后放电的参数，如频率和持续时间的后放电，不同的地区。N-甲基-D-天冬氨酸（NMDA）和非NMDA受体拮抗剂或GABA（A）受体拮抗剂的混合物完全阻断后放电。在后放电过程中的本地GABA的应用引起去极化，而不是超极化。此外，后放电的反转电位约为-40 mV。与此相反，强直刺激导致DG脑片偶尔出现后放电样活动，这种活动可被非NMDA或GABA（A）受体拮抗剂阻断。这些结果表明，通过兴奋性GABA能和谷氨酸能传输介导的后放电可能是共同的，但在海马和皮层的个别局部网络的调制不同。(c)2005年Elsevier爱尔兰有限公司和日本神经科学学会。All rights reserved.
Strong tetanization induces synchronous membrane potential oscillations (seizure-like afterdischarge) in mature pyramidal cells of the hippocampal CA1 region. To investigate whether local networks in other brain regions can generate such an afterdischarge independently, we studied the inducibility of afterdischarge in individual 'isolated slices' of the rat hippocampal CA1 and CA3 regions, dentate gyrus (DG), entorhinal cortex (EC), and temporal cortex (TC) using intracellular and extracellular recordings. The strong tetanization constantly induced afterdischarges in the CA1 and CA3 pyramidal cells as well as in the EC and TC superficial principal cells. However, parameters of the afterdischarges, such as the frequency and duration of afterdischarges, varied among the regions. A mixture of N-methyl-D-aspartate (NMDA) and non-NMDA receptor antagonists or a GABA(A) receptor antagonist completely blocked the afterdischarges. Local GABA application during the afterdischarge elicited depolarization, rather than hyperpolarization. Moreover, reversal potentials of the afterdischarge were around -40 mV. In contrast, the tetanization resulted in occasional afterdischarge-like activities in DG slices, which were blocked by the non-NMDA or GABA(A) receptor antagonist. These findings suggest that the afterdischarges mediated through the excitatory GABAergic and glutamatergic transmissions might be common to, but be modulated differently by individual local networks in the hippocampus and cortex. (c) 2005 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.