Interspecies antigenic determinants of the reverse transcriptases and p30 proteins of mammalian type C viruses
Interspecies antigenic determinants of the reverse transcriptases and p30 proteins of mammalian type C viruses
复制标题
哺乳动物C型病毒逆转录酶和p30蛋白的种间抗原决定簇
作者：
C. Sherr;L. Fedele;R. Benveniste;G. Todaro
本文用放射免疫沉淀法和竞争性放射免疫分析法研究了从几种哺乳动物中分离的C型病毒的主要内部结构蛋白（p30）。两种方法都可以证明三组抗原性可区分的种间决定簇。啮齿类动物来源的病毒（小鼠和大鼠）共有的一组决定簇可以在猫白血病病毒中容易地检测到，但在其他C型病毒组中不能。鼠病毒的p30蛋白还含有与传染性灵长类病毒和内源性猪病毒中的抗原决定簇相关的第二组离散的抗原决定簇，但在猫白血病病毒组中未检测到。狒狒和家猫内源性病毒的p30蛋白共享第三组交叉反应决定簇，而在从其他动物物种分离的C型病毒中未检测到。用抗血清对这些相同的C型病毒组的逆转录酶进行酶抑制研究，结果显示与p30蛋白相同的免疫交叉反应模式。从遗传上远距离的动物中分离的C型病毒的同源蛋白之间的抗原交叉反应可能反映了C型病毒跨越种属屏障的传播。
The major internal structural proteins (p30) of type C viruses isolated from several mammalian species were studied by radioimmunoprecipitation and competitive radioimmunoassays. Three antigenically distinguishable sets of interspecies determinants could be demonstrated by both methods. One set of determinants shared by viruses of rodent origin (mouse and rat) can be detected readily in feline leukemia viruses but not in other type C viral groups. The p30 proteins of murine viruses also contain a second discrete set of antigenic determinants related to those in infectious primate viruses and endogenous porcine viruses, but not detected in the feline leukemia virus group. The p30 proteins of endogenous viruses of baboons and domestic cats share yet a third set of cross-reactive determinants not detected in type C viruses isolated from other species of animals. Enzyme inhibition studies performed with antisera raised toward the reverse transcriptases of these same groups of type C viruses showed the same patterns of immunological cross-reactions as observed with p30 proteins. The antigenic cross-reactions between the homologous proteins of type C virus isolated from genetically distant animals may reflect transmission of type C viruses across species barriers.