Intrauterine transmission of human T-cell leukemia virus type I in rats
Intrauterine transmission of human T-cell leukemia virus type I in rats
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人类 T 细胞白血病病毒 I 型在大鼠体内的宫内传播
DOI：
10.1128/jvi.69.2.1302-1305.1995
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发表时间：
1995
影响因子：
5.4
通讯作者：
Masanao Miwa
中科院分区：
文献类型：
--
作者：
Mitsuo Hori;Y. Ami;S. Kushida;Mikirou Kobayashi;Kazuhiko Uchida;Tsukasa Abe;Masanao Miwa
为了分析宫内传播，将MT-2细胞（一种产生人T细胞白血病病毒I型（HTLV-I）的人T细胞系）注射到8只妊娠F344大鼠中，并在妊娠第23天进行剖宫产。用PCR方法从其中一个胎儿的肝脏和脾脏中检测到HTLV-Ⅰ型前病毒。此外，71个后代从其余7个母鼠剖腹产分娩，并由7个正常大鼠饲养。剖宫产术后4周，71只子代中有2只外周血单个核细胞中检测到HTLV-Ⅰ型前病毒。这些结果首次表明HTLV-I的宫内传播。为了证实产后传播，MT-2细胞注射到母鼠在分娩后24小时内，并由该母鼠培育6个后代。HTLV-I前病毒检测外周血单个核细胞的所有6个后代。该动物模型可用于分析和预防HTLV-I的母婴传播。
To analyze intrauterine transmission, MT-2 cells, a human T-cell line producing human T-cell leukemia virus type I (HTLV-I), were injected into eight pregnant F344 rats, and cesarean section was performed at day 23 of pregnancy. HTLV-I provirus was detected by PCR in the liver and spleen taken from one of the eight fetuses. Moreover, 71 offspring were delivered by cesarean section from the remaining seven dams and fostered by seven normal rats. HTLV-I provirus was detected in peripheral blood mononuclear cells in 2 of the 71 offspring 4 weeks after cesarean section. These results indicate for the first time the intrauterine transmission of HTLV-I. To confirm the postnatal transmission, MT-2 cells were injected into a dam within 24 h after delivery, and six offspring were fostered by this dam. HTLV-I provirus was detected in peripheral blood mononuclear cells of all six offspring. This animal model may be useful for analysis and prevention of mother-to-child transmission of HTLV-I.
影响因子：
--
作者：
G. Pfeifer;A. Riggs
通讯作者：
G. Pfeifer;A. Riggs