Investigation of Prx1 protein expression provides evidence for conservation of cardiac-specific posttranscriptional regulation in vertebrates
Investigation of Prx1 protein expression provides evidence for conservation of cardiac-specific posttranscriptional regulation in vertebrates
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DOI：
10.1002/dvdy.1198
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发表时间：
2001-11-01
影响因子：
2.5
通讯作者：
Kern, MJ
中科院分区：
文献类型：
--
作者：
Chesterman, ES;Gainey, GD;Kern, MJ
基因打靶实验已经确定，同源框基因PRX1对于正常的颅面、肢体和血管发育是必不可少的。虽然它的RNA表达模式已经很好地建立了，但PRX1蛋白在发育中的胚胎中的表达还没有被检测到。制备了一种新的PRX1抗体，用于确定发育中的小鼠胚胎中正常的PRX1蛋白表达模式。在颅面和肢体间充质中，PRX1蛋白的表达与先前发表的RNA定位数据一致。然而，在心脏组织中发现了显著的差异。尽管存在PRX1转录本，但在小鼠胚胎和成年心脏中检测不到PRX1蛋白。这些数据表明PRX1的表达是转录后调控的。在鸡胚胎心脏中也观察到PRX1转录本的存在和可检测到的蛋白的缺失之间的差异，这表明脊椎动物的调节机制是保守的。这一观察为为什么Prx基因缺失的小鼠没有心脏畸形提供了新的解释。(C)2001年Wiley-Liss，Inc.
Gene targeting experiments have defined that the homeobox gene Prx1 is essential for normal craniofacial, limb, and vascular development. Although its RNA expression pattern is well established, Prx1 protein expression in the developing embryo has not been examined. A novel Prx1 antibody was produced to define the normal Prx1 protein expression pattern in the developing mouse embryo. In craniofacial and limb mesenchyme, Prx1 protein expression is consistent with previously published data on RNA localization. However, a remarkable discrepancy was found in cardiac tissue. Prx1 protein is undetectable in the murine embryonic and adult heart, despite the presence of Prx1 transcripts. These data demonstrate that Prx1 expression is posttranscriptionally regulated. This discrepancy between the presence of Prx1 transcript and the absence of detectable protein was also observed in embryonic chick heart, suggesting conservation of the regulatory mechanism in vertebrates. This observation provides a new explanation of why the Prx null mice lack cardiac malformations. (C) 2001 Wiley-Liss, Inc.