Systems biology discoveries using non-human primate pluripotent stem and germ cells: novel gene and genomic imprinting interactions as well as unique expression patterns.
Systems biology discoveries using non-human primate pluripotent stem and germ cells: novel gene and genomic imprinting interactions as well as unique expression patterns.
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DOI：
10.1186/scrt24
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发表时间：
2010-08-05
影响因子：
7.5
通讯作者：
Schatten G
中科院分区：
文献类型：
--
作者：
Ben-Yehudah A;Easley CA 4th;Hermann BP;Castro C;Simerly C;Orwig KE;Mitalipov S;Schatten G
多能干细胞的研究引起了生物学和医学界的极大兴趣。了解生物学决定的基本原理，包括什么允许细胞保持多能性，即其自我更新并因此保持不朽的能力，或分化为多种类型的细胞，具有极其重要的意义。在临床应用方面，包括胚胎干细胞和成人干细胞在内的多潜能细胞已被提议用于治疗许多人类疾病和障碍的细胞替代疗法，包括阿尔茨海默氏症、帕金森氏症、脊髓损伤和糖尿病。在这些疗法的使用中，一个挑战是理解允许维持多能性和控制特定分化为所需功能靶细胞的机制。由于监管限制和生物学上的可行性，有许多关键的研究是不可能使用来自人类的多潜能干细胞(PSCs)进行的(例如，从有血统和有生育能力的捐赠者获得的原始胚胎中直接比较近亲繁殖的胚胎干细胞小组；对父母和后代PSCs及其相同的分化组织进行基因组分析；用于多能性测试的种内嵌合体分析；等等)。然而，来自非人类灵长类动物的PSCs正在进行研究，以弥合在小鼠身上的发现和适当临床评估所需的重要信息之间的知识差距。在这篇综述中，我们考虑了利用灵长类多能干细胞和生殖细胞的系统生物学方法发现的具有独特表达和印记模式的mRNAs和新基因。
The study of pluripotent stem cells has generated much interest in both biology and medicine. Understanding the fundamentals of biological decisions, including what permits a cell to maintain pluripotency, that is, its ability to self-renew and thereby remain immortal, or to differentiate into multiple types of cells, is of profound importance. For clinical applications, pluripotent cells, including both embryonic stem cells and adult stem cells, have been proposed for cell replacement therapy for a number of human diseases and disorders, including Alzheimer's, Parkinson's, spinal cord injury and diabetes. One challenge in their usage for such therapies is understanding the mechanisms that allow the maintenance of pluripotency and controlling the specific differentiation into required functional target cells. Because of regulatory restrictions and biological feasibilities, there are many crucial investigations that are just impossible to perform using pluripotent stem cells (PSCs) from humans (for example, direct comparisons among panels of inbred embryonic stem cells from prime embryos obtained from pedigreed and fertile donors; genomic analysis of parent versus progeny PSCs and their identical differentiated tissues; intraspecific chimera analyses for pluripotency testing; and so on). However, PSCs from nonhuman primates are being investigated to bridge these knowledge gaps between discoveries in mice and vital information necessary for appropriate clinical evaluations. In this review, we consider the mRNAs and novel genes with unique expression and imprinting patterns that were discovered using systems biology approaches with primate pluripotent stem and germ cells.
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DOI：
10.1089/clo.2009.0004
发表时间：
2009-06-01
期刊：
CLONING AND STEM CELLS
影响因子：
--
作者：
Chung, Young;Bishop, Colin E.;Lanza, Robert
通讯作者：
Lanza, Robert
影响因子：
5.2
作者：
Bucay, Nathan;Yebra, Mayra;Montgomery, Anthony M. P.
通讯作者：
Montgomery, Anthony M. P.
影响因子：
6.1
作者：
Chen, H. -F.;Kuo, H. -C.;Ho, H-N.
通讯作者：
Ho, H-N.
DOI：
10.1101/sqb.2008.73.027
发表时间：
2008
期刊：
Cold Spring Harbor symposia on quantitative biology
影响因子：
--
作者：
Cole MF;Young RA
通讯作者：
Young RA
影响因子：
3.6
作者：
Byrne, James A.;Mitalipov, Shoukhrat M.;Wolf, Don P.
通讯作者：
Wolf, Don P.