Early effects of acute γ‐radiation on vascular arterial tone
Early effects of acute γ‐radiation on vascular arterial tone
复制标题
急性γ辐射对血管动脉张力的早期影响
DOI：
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发表时间：
1998
期刊：
影响因子：
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通讯作者：
J. Verdetti
中科院分区：
文献类型：
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作者：
V. Bourlier;M. Diserbo;M. Joyeux;C. Ribuot;Eric Multon;P. Gourmelon;J. Verdetti
1为了确定辐射对血管功能的急性效应，将大鼠主动脉环固定在器官浴槽中进行等长张力测量并进行辐射（60 Co，1戈伊min-1，15 min）。 2辐射对非收缩或去内皮血管没有影响，但导致（-）-去氧肾上腺素（1 μM）-预收缩主动脉环的血管张力立即且可逆地增加。 在照射开始后约5分钟，张力达到平台。3最大辐射诱导收缩发生在乙酰胆碱（ACh）（1 μM）松弛的主动脉环上。 在此条件下，添加还原过氧化氢的过氧化氢酶（1000 u ml−1）和清除羟基自由基的DMSO（0.1% v/v）对张力水平没有影响，而超氧化物歧化酶（SOD）（100 u ml−1），超氧阴离子清除剂，减少了观察到的收缩。 在存在吲哚美辛（10 μM）（一种环加氧酶阻断剂）的情况下获得了类似的结果。 4用一氧化氮合酶抑制剂Nω-硝基-L-精氨酸甲酯（L-NAME）（10-100 μM）预处理环抑制辐射诱导的收缩。 [5]这种效应是剂量率依赖性的，甚至在非常低的剂量率（0.06戈伊min-1）下也会发生。 6目前的结果表明，γ辐射诱导瞬时血管张力增加，这是内皮和剂量率依赖性的。这种作用是（i）最大的一氧化氮（NO）时产生，（ii）大大减少SOD和（iii）抑制L-NAME，表明NO和超氧阴离子之间的复合物的主要参与。
1 To determine the acute effects of irradiation on the functionality of vessel, rat aortic rings were mounted in an organ bath for isometric tension measurements and irradiated (60Co, 1 Gy min−1, 15 min). 2 Irradiation, which is without effect on non‐contracted or endothelium‐denuded vessels, led to an immediate and reversible increase in vascular tone on (−)‐phenylephrine (1 μM)‐precontracted aortic rings. The tension reached a plateau about 5 min after the beginning of irradiation. 3 The maximal radiation‐induced contraction occurred on aortic rings relaxed by acetylcholine (ACh) (1 μM). In this condition, the addition of catalase (1000 u ml−1), which reduces hydrogen peroxide, and DMSO (0.1% v/v), which scavenges hydroxyl radical, had no influence on tension level while superoxide dismutase (SOD) (100 u ml−1), a superoxide anion scavenger, reduced the observed contraction. A similar result was obtained in the presence of indomethacin (10 μM), a cyclo‐oxygenase blocker. 4 Pretreatment of rings with the nitric oxide synthase inhibitor, Nω‐nitro‐L‐arginine methyl ester (L‐NAME) (10–100 μM) inhibited the radiation‐induced contraction. 5 This effect was dose rate‐dependent and even occurred for a very low dose rate (0.06 Gy min−1). 6 The present results indicate that γ‐radiation induces an instantaneous vascular tone increase that is endothelium and dose rate‐dependent. This effect is (i) maximal when nitric oxide (NO) is produced, (ii) greatly reduced by SOD and (iii) inhibited by L‐NAME, suggesting a major involvement of complexes between NO and superoxide anion.
影响因子：
7.4
作者：
S. J. Elliott;S. Koliwad
通讯作者：
S. J. Elliott;S. Koliwad
过氧亚硝酸盐是超氧化物和一氧化氮的产物，可以使狗的冠状血管舒张。
DOI：
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发表时间：
1994
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
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作者：
Liu,S;Beckman,JS;Ku,DD
通讯作者：
Ku,DD