Dorsal Root Ganglia Sensory Neuronal Cultures: a tool for drug discovery for peripheral neuropathies.
Dorsal Root Ganglia Sensory Neuronal Cultures: a tool for drug discovery for peripheral neuropathies.
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DOI：
10.1517/17460440903266829
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发表时间：
2009-10-01
影响因子：
6.3
通讯作者：
Höke A
中科院分区：
文献类型：
--
作者：
Melli G;Höke A
周围神经疾病影响世界各地的许多人，由多种疾病引起或与之相关，包括遗传和后天疾病。目前的治疗方法是针对症状控制，因为不存在有效的再生治疗。主要的挑战是，导致远端轴突变性的机制在很大程度上是未知的，而远端轴突变性是所有周围神经疾病的共同特征。目的探讨背根神经节(DRG)来源的感觉神经元培养系统在评估周围神经病的发病机制以及检测和验证潜在治疗化合物方面的作用和特点。完成了对最近文献的全面回顾，并选择了在不同的周围神经病模型中使用DRG神经元的例子，以突出这些培养物的实用性。利用DRG神经元培养建立了许多不同的周围神经疾病的有用模型，并研究了潜在的治疗靶点，但到目前为止，还没有任何潜在的治疗化合物在临床试验中成功。近年来，重点已转向评估轴突变性作为主要结果衡量标准，倡导从表型药物筛选开始，然后在初级复杂共培养和动物模型中验证的药物开发策略。
Peripheral neuropathies affect many people worldwide and are caused by or associated with a wide range of conditions, both genetic and acquired. Current therapies are directed at symptomatic control because no effective regenerative treatment exists. Primary challenge is that mechanisms that lead to distal axonal degeneration, a common feature of all peripheral neuropathies, are largely unknown. To address the role and specific characteristics of dorsal root ganglia (DRG) derived sensory neuron culture system as a useful model in evaluating the pathogenic mechanisms of peripheral neuropathies and examination and validation of potential therapeutic compounds. A thorough review of the recent literature was completed and select examples of the use of DRG neurons in different peripheral neuropathy models were chosen to highlight the utility of these cultures. Many useful models of different peripheral neuropathies have been developed using DRG neuronal culture and potential therapeutic targets have been examined, but so far none of the potential therapeutic compounds have succeeded in clinical trials. In recent years, focus has changed to evaluation of axon degeneration as the primary outcome measure advocating a drug development strategy starting with phenotypic drug screening, followed by validation in primary complex co-cultures and animal models.
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影响因子：
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