Nitrate as a potential prebiotic for the oral microbiome
Nitrate as a potential prebiotic for the oral microbiome
复制标题
DOI：
10.1038/s41598-020-69931-x
复制
发表时间：
2020-07-30
影响因子：
4.6
通讯作者：
Mira, Alex
中科院分区：
文献类型：
--
作者：
Rosier, B. T.;Buetas, E.;Mira, Alex
唾液腺主动浓缩血浆硝酸盐，导致富含硝酸盐的蔬菜餐后唾液硝酸盐浓度高（5 - 8 mM）。硝酸盐是一种生态因子，可引起多种微生物群落结构和功能的快速变化，但对口腔微生物群的影响尚未阐明。为了测试这一点，收集了12名健康供体的唾液，在有和没有6.5 mM硝酸盐的情况下生长体外生物膜。在生物膜形成的5小时（大多数硝酸盐还原）和9小时（所有硝酸盐还原）时取样用于铵、乳酸盐和pH测量，以及16S rRNA基因Illumina测序。硝酸盐对生物膜的生长没有显著影响，但减少了乳酸的产生，同时增加了观察到的铵的产生和pH值（所有p
The salivary glands actively concentrate plasma nitrate, leading to high salivary nitrate concentrations (5-8 mM) after a nitrate-rich vegetable meal. Nitrate is an ecological factor that can induce rapid changes in structure and function of polymicrobial communities, but the effects on the oral microbiota have not been clarified. To test this, saliva of 12 healthy donors was collected to grow in vitro biofilms with and without 6.5 mM nitrate. Samples were taken at 5 h (most nitrate reduced) and 9 h (all nitrate reduced) of biofilm formation for ammonium, lactate and pH measurements, as well as 16S rRNA gene Illumina sequencing. Nitrate did not affect biofilm growth significantly, but reduced lactate production, while increasing the observed ammonium production and pH (all p