HSAF-induced antifungal effects in Candida albicans through ROS-mediated apoptosis.
HSAF-induced antifungal effects in Candida albicans through ROS-mediated apoptosis.
复制标题
HSAF 通过 ROS 介导的细胞凋亡在白色念珠菌中诱导抗真菌作用。
作者：
Ding Y;Li Z;Li Y;Lu C;Wang H;Shen Y;Du L
热稳定抗真菌因子（HSAF）属于多环四酸酯大环内酰胺类（PTM），对多种真菌病原体有抑制作用，对白色念珠菌（C。白色念珠菌）。本研究发现HSAF通过诱导活性氧的产生诱导白念珠菌SC5314细胞凋亡。然而，我们证实了HSAF对念珠菌引起的念珠菌病的疗效。与载体相比，HSAF在体内鼠模型中显著提高了存活率并降低了真菌负荷。通过分子动力学模拟，揭示了HSAF与C.白色念珠菌。本研究首次发现PTMs通过在C.白色念珠菌及其作为杀真菌剂的新试剂的潜力。
Heat-stable antifungal factor (HSAF) belongs to polycyclic tetramate macrolactams (PTMs), which inhibits many fungal pathogens and is effective in inhibiting Candida albicans (C. albicans). In this study, we found that HSAF induced the apoptosis of Candida albicans SC5314 through inducing the production of reactive oxygen species (ROS). Nevertheless, we validated the efficacy of HSAF against candidiasis caused by C. albicans in a murine model in vivo, and HSAF significantly improved survival and reduced fungal burden compared to vehicles. A molecular dynamics (MD) simulation was also investigated, revealing the theoretical binding mode of HSAF to the β-tubulin of C. albicans. This study first found PTMs-induced fungal apoptosis through ROS accumulation in C. albicans and its potential as a novel agent for fungicides.