Large-scale gene expression profiling reveals physiological response to deletion of chaperone dnaKJ in Escherichia coli
Large-scale gene expression profiling reveals physiological response to deletion of chaperone dnaKJ in Escherichia coli
复制标题
大规模基因表达谱揭示了大肠杆菌中伴侣蛋白 dnaKJ 缺失的生理反应
DOI：
10.1016/j.micres.2016.03.001
复制
发表时间：
2016-01-01
影响因子：
6.7
通讯作者：
Zhou, Qiming
中科院分区：
文献类型：
--
作者：
Fan, Dongjie;Liu, Chuanpeng;Zhou, Qiming
分子伴侣DNAK及其辅助分子DNAJ在帮助新生多肽折叠、阻止蛋白质聚集和维持细胞蛋白质动态平衡等方面发挥着重要作用。利用DNA微阵列技术检测与dna KJ缺失相关的体内整体转录变化，以阐明dna KJ在转录水平上的作用。基因芯片分析和生物信息学分析表明，一些伴侣和蛋白酶基因、胁迫相关基因和参与三羧酸循环和氧化磷酸化的基因表达上调，而各种转运蛋白基因、戊糖磷酸途径和转录调控相关基因表达下调。本研究首次系统分析了dna KJ基因缺失对体内转录水平的影响。脂肪酸甲酯分析表明，不饱和脂肪酸含量急剧增加，亚细胞定位预测分析显示，内膜蛋白基因转录显著减少，这可能是导致三角形dna KJ株细胞形态异常和对某些抗生素敏感性增加的原因。(C)2016年爱思唯尔股份有限公司。版权所有
Chaperone DnaK and its co-chaperone DnaJ plays various essential roles such as in assisting in the folding of nascent peptides, preventing protein aggregation and maintaining cellular protein homeostasis. Global transcriptional changes in vivo associated with deletion of dnaKJ were monitored using DNA microarray to elucidate the role of DnaKJ at the transcriptional level. Microarray profiling and bioinformatics analysis revealed that a few chaperone and protease genes, stress-related genes and genes involved in the tricarboxylic acid cycle and oxidative phosphorylation were up-regulated, whereas various transporter genes, pentose phosphate pathway and transcriptional regulation related genes were down-regulated. This study is the first to systematically analyze the alterations at the transcriptional level in vivo in deletion of dnaKJ. Fatty acid methyl esters analysis indicated that the amount of unsaturated fatty acid sharply increased and subcellular location prediction analysis showed a marked decrease in transcription of inner-membrane protein genes, which might have triggered the development of aberrant cell shape and susceptibility for some antibiotics in the triangle dnaKJ strain. (C) 2016 Elsevier GmbH. All rights reserved