Synthesis and enzymatic stability of phosphodiester-linked peptide - Oligonucleotide hybrids
Synthesis and enzymatic stability of phosphodiester-linked peptide - Oligonucleotide hybrids
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DOI：
10.1021/bc970051u
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发表时间：
1997-11-01
影响因子：
4.7
通讯作者：
Grandas, A
中科院分区：
文献类型：
--
作者：
Robles, J;Maseda, M;Grandas, A
核肽Ac-Tyr（p（3 ′）dACGT）-Ala-Phe-Gly-NH 2、Ac-Thr（p（3 ′）dACGT）-Ala-Phe-Gly-OH、Ac-Ser（p（3 ′）dACGT）-Ala-Phe-Gly-OH和Phac-Hse（p（3 ′）dACGT）-Ala-Phe-Gly-OH，其中四脱氧核糖核苷酸的3 ′-末端通过磷酸二酯键与羟基化氨基酸连接，使用分步固相法合成了一系列的核酸外切酶，以研究连接氨基酸对它们对3 '-核酸外切酶的稳定性的影响。对蛇毒磷酸二酯酶处理后的反应产物进行HPLC分析，结果表明，氨基酸-核苷键的不稳定性按Thr < Ser < Hse < Tyr的顺序增加。
Nucleopeptides Ac-Tyr(p(3')dACGT)-Ala-Phe-Gly-NH2, Ac-Thr(p(3')dACGT)-Ala-Phe-Gly-OH, Ac-Ser(p(3')dACGT)-Ala-Phe-Gly-OH, and Phac-Hse(p(3')dACGT)-Ala-Phe-Gly-OH, in which the 3'-end of a tetradeoxyribonucleotide is linked by a phosphodiester bond to a hydroxylated amino acid, were synthesized using a stepwise solid-phase methodology to study the influence of the linking amino acid on their stability to 3'-exonucleases. HPLC analysis of the reaction crudes after treatment of each nucleopeptide with snake venom phosphodiesterase showed that the lability of the amino acid-nucleoside linkage increases in the order Thr < Ser < Hse < Tyr.