Improving Erythritol Production of Aureobasidium pullulans from Xylose by Mutagenesis and Medium Optimization
Improving Erythritol Production of Aureobasidium pullulans from Xylose by Mutagenesis and Medium Optimization
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DOI：
10.1007/s12010-016-2127-3
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发表时间：
2016-10-01
影响因子：
3
通讯作者：
Xiao, Dongguang
中科院分区：
文献类型：
--
作者：
Guo, Jian;Li, Junxia;Xiao, Dongguang
木糖是一种含量第二丰富的糖类，其生物转化一直是近年来研究的热点。本研究对出芽短梗霉CGMCC3.0837进行了紫外线诱变，以提高其木糖转化率。获得的突变株ER35的赤霉醇产量比出发菌株提高了50.92%(17.28g/L)。采用响应曲面法对培养基组分进行了优化。酵母膏、磷酸二氢钾和柠檬酸是影响赤藓糖醇合成的关键因素，其最适浓度分别为17.82、0.76和6.60g/L。在优化条件下，摇瓶发酵的赤藓糖醇产量为31.75g/L，5-L发酵罐的产量为31.42g/g。ER35对木糖母液也表现出良好的消耗能力，最终赤藓糖醇产量为26.35g/L。这为研究出芽孢杆菌以木糖为碳源生产赤霉醇的潜力提供了依据。
The biotransformation of xylose, the second most abundant sugar, has been a hot topic in recent years. In this work, Aureobasidium pullulans CGMCC3.0837 was subjected to UV mutagenesis to improve its erythritol production from xylose. The erythritol production of the obtained mutant ER35 was 50.92 % (17.28 g/L) higher than that of the parent strain. Response surface methodology was applied to optimize the medium composition. Yeast extract, KH2PO4, and citric acid were the key factors influencing erythritol synthesis, and the optimal concentrations were 17.82, 0.76, and 6.60 g/L, respectively. Under the optimized conditions, 31.75 and 31.42 g/L erythritol were obtained in shake flasks and in a 5-L fermentor, respectively. ER35 also showed a good consuming ability on xylose mother liquor with a final erythritol production of 26.35 g/L. This report provided insights into the potential of A. pullulans for the production of erythritol using xylose as a carbon source.