A Cytoprotective and Degradable Metal-Polyphenol Nanoshell for Single-Cell Encapsulation
A Cytoprotective and Degradable Metal-Polyphenol Nanoshell for Single-Cell Encapsulation
复制标题
DOI：
10.1002/anie.201405905
复制
发表时间：
2014-11-10
影响因子：
16.6
通讯作者：
Choi, Insung S.
中科院分区：
文献类型：
--
作者：
Park, Ji Hun;Kim, Kyunghwan;Choi, Insung S.
单细胞包裹承诺对被包裹的细胞进行细胞保护以对抗致命的压力源，这让人想起自然界中的孢子形成过程。然而，用于化学模拟萌发过程的细胞相容性方法（即，尽管壳降解对于功能性细胞的实际应用以及对于基于单细胞的生物学是关键的，但壳降解（按需降解）一直是难以捉摸的。我们报告说，人工壳，由单宁酸（TA）和铁III，对个别酿酒酵母可控地按需降解，同时保护酵母从多个外部侵略者，包括UV-C照射，溶解酶，和银纳米粒子。细胞分裂被TA-Fe-III壳抑制，但在壳降解后完全恢复。TA-Fe-III壳的形成将提供用于实现孢子形成和萌发的化学形式的通用工具。
Single-cell encapsulation promises the cytoprotection of the encased cells against lethal stressors, reminiscent of the sporulation process in nature. However, the development of a cytocompatible method for chemically mimicking the germination process (i.e., shell degradation on-demand) has been elusive, despite the shell degradation being pivotal for the practical use of functional cells as well as for single cell-based biology. We report that an artificial shell, composed of tannic acid (TA) and Fe-III, on individual Saccharomyces cerevisiae controllably degrades on-demand, while protecting the yeast from multiple external aggressors, including UV-C irradiation, lytic enzymes, and silver nanoparticles. Cell division is suppressed by the TA-Fe-III shell, but restored fully upon shell degradation. The formation of a TA-Fe-III shell would provide a versatile tool for achieving the chemical version of sporulation and germination.