Schizophyllum commune β-glucan: Effect on interleukin-10 expression induced by lipopolysaccharide from periodontopathic bacteria.
Schizophyllum commune β-glucan: Effect on interleukin-10 expression induced by lipopolysaccharide from periodontopathic bacteria.
复制标题
裂褶菌 β-葡聚糖：对牙周病细菌脂多糖诱导的白细胞介素 10 表达的影响。
DOI：
10.1016/j.carbpol.2020.117285
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发表时间：
2021
影响因子：
11.2
通讯作者：
Wataru Ariyoshi
中科院分区：
文献类型：
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作者：
Chuencheewit Thongsiri;Yoshie Nagai-Yoshioka;Ryota Yamasaki;Yoshiyuki Adachi;Michihiko Usui;Keisuke Nakashima;Tatsuji Nishihara;Wataru Ariyoshi
β-葡聚糖是一种有效的免疫调节剂，通过dectin-1作为主要受体影响先天和适应性免疫反应。在本研究中，我们研究了裂藻（schizophyllum commune， SPG） β-葡聚糖对小鼠巨噬细胞中由放线菌脂多糖（LPS）诱导的白细胞介素-10 （IL-10）表达的生物学效应（J774.1）。SPG和dectin-1相互作用上调lps诱导的IL-10表达。SPG对IL-10表达的调节作用依赖于LPS诱导的核因子-κB （NF-κBα）通路核易位活性的延长。我们还发现，通过激活脾酪氨酸激酶（Syk）， SPG启动刺激了lps诱导的丝裂原和应激激活蛋白激酶1 （MSK1）和camp响应元件结合蛋白（CREB）的磷酸化，随后是IL-10的上调。我们的数据表明SPG增强了小鼠巨噬细胞的抗炎反应，这可能有助于创建牙周病治疗的干预措施。
β-glucans are potent immunomodulators, with effects on innate and adaptive immune responses via dectin-1 as the main receptor. In this study, we investigated the biological effect ofβ-glucan fromSchizophyllum commune, called Schizophyllan (SPG) on Interleukin-10 (IL-10) expression induced by a lipopolysaccharide (LPS) fromAggregatibacter actinomycetemcomitansin murine macrophages (J774.1). SPG and dectin-1 interaction up-regulates LPS-induced IL-10 expression. The regulative effect of SPG on IL-10 expression is dependent on prolongation of nuclear translocation activity of nuclear factor-kappa B (NF-κBα) pathway induced by LPS. We also found that LPS-induced phosphorylation of mitogen- and stress-activated protein kinase 1 (MSK1) and cAMP-responsive-element-binding protein (CREB), followed by up-regulation of IL-10, was stimulated by SPG priming via activation of the spleen tyrosine kinase (Syk). Our data indicate that SPG augments the anti-inflammatory response in murine macrophages which can be useful to create an intervention for periodontal disease treatment.